Coix seed oil containing 13 glycerides, and pharmaceutical preparation and use thereof

ABSTRACT

The present invention relates to  Coix  seed oil extracted from  Semen Coicis , pharmaceutical preparations thereof, and the use thereof in the treatment of tumors and inflammation. Specifically, the  Coix  seed oil contains 5 diglyceride and 8 triglyceride ingredients in the following mass percentages: 1,3-diolein 0.40-0.58%, 1-linolein-3-olein 0.91-1.31%, 1,2-diolein 0.24-0.35%, 1-olein-2-linolein 0.66-0.95%, 1,2-dilinolein 0.33-0.47%, trilinolein 4.87-6.99%, 1-olein-2,3-dilinolein 13.00-18.69%, 1-palmitin-2,3-dilinolein 5.25-7.54%, 1,3-diolein-2-linolein 13.23-19.02%, 1-palmitin-2-linolein-3-olein 10.26-14.75%, 1,3-dipalmitin-2-linolein 2.28-3.28%, triolein 14.44-20.76% and 1-palmitin-2,3-diolein 8.06-11.58%.

CROSS REFERENCE TO RELATED PATENT APPLICATION

The present application claims a priority of the Chinese patentapplication CN201410342342.1 with filing date Jul. 18, 2014, whichapplication is incorporated herein by reference.

FIELD OF THE INVENTION

The present invention relates to the pharmaceutical field, specifically,the present invention relates to Coix seed oil, pharmaceuticalpreparations thereof, the process for the preparation of same and theuse thereof in the treatment of tumors and inflammation.

BACKGROUND OF THE INVENTION

Coix seeds are dried ripe seeds of Coix lacryma-jobi L. var ma-yuen(Roman.), Stapf, a genus of plant in the Poaceae family. It is adampness-eliminating drug and has been used as a medicinal and edibleplant for a long time. Modern researches have found that Coix seeds havemany pharmacological effects, such as analgesic anti-inflammatory,immunomodulatory, anti-ulcer, hypolipidemic and anti-obesity effects. Inrecent years, researchers around the world have studied the chemicalcomposition of the Coix seed by using TLC, HPLC-MS, GC, etc., and founda variety of active ingredients in it, including coixenolide,triglycerides, fatty acids, lactams, coix lactones, saccharides, sterolsand triterpenoids. Among them, esters are the first discoveredcomponents having anti-tumor activities and the most reported chemicalcomposition attracting the most attention. Kanglaite injection, in whichthe active ingredient is Coix seed oil, has been widely used in presentChinese clinical applications, but the Coix seed oil used in theKanglaite injection comprises complex components. In addition totriglycerides, it also contains monoglycerides, diglycerides and fattyacid esters, etc. This will inevitably be a great challenge for thequality control in the practical production process and the safety inclinical applications.

In the present invention, the raw material Coix seed powder underwentsupercritical carbon dioxide extraction, basification, neutral aluminapurification and kaolin purification, etc., to afford an effective part,Coix seed oil. With the active ingredients' isolation andidentification, it is determined that the Coix seed oil comprises mainlyeight triglyceride components and 5 diglyceride components. Furtherdetermination of physicochemical constants has confirmed the optimalacid value, iodine value, saponification value, refractive index andspecific gravity, etc. The use of the Coix seed oil of the invention inmedication has advantages such as the confirmed composition ofingredients, ensuring quality stability in every batch in the industrialproduction.

SUMMARY OF THE INVENTION

The first aspect of the invention is to provide a Coix seed oilextracted from Semen Coilis. The Coix seed oil contains 5 diglycerideand 8 triglyceride ingredients in the following mass percentages:1,3-diolein 0.40-0.58%, 1-linolein-3-olein 0.91-1.31%, 1,2-diolein0.24-0.35%, 1-olein-2-linolein 0.66-0.95%, 1,2-dilinolein 0.33-0.47%,trilinolein 4.87-6.99%, 1-olein-2,3-dilinolein 13.00-18.69%,1-palmitin-2,3-dilinolein 5.25-7.54%, 1,3-diolein-2-linolein13.23-19.02%, 1-palmitin-2-linolein-3-olein 10.26-14.75%,1,3-dipalmitin-2-linolein 2.28-3.28%, triolein 14.44-20.76% and1-palmitin-2,3-diolein 8.06-11.58%.

Preferably, mass percentage contents of the above 5 diglyceride and 8triglyceride ingredients are: 1,3-diolein 0.45-0.55%, 1-linolein-3-olein1.03-1.25%, 1,2-diolein 0.27-0.33%, 1-olein-2-linolein 0.75-0.91%,1,2-dilinolein 0.37-0.45%, trilinolein 5.47-6.69%,1-olein-2,3-dilinolein 14.63-17.88%, 1-palmitin-2,3-dilinolein5.90-7.21%, 1,3-diolein-2-linolein 14.88-18.19%,1-palmitin-2-linolein-3-olein 11.55-14.11%, 1,3-dipalmitin-2-linolein2.57-3.14%, triolein 16.25-19.86% and 1-palmitin-2,3-diolein9.07-11.08%.

More preferably, mass percentage contents of the above 5 diglyceride and8 triglyceride ingredients are: 1,3-diolein 0.49-0.51%,1-linolein-3-olein 1.12-1.16%, 1,2-diolein 0.29-0.31%,1-olein-2-linolein 0.81-0.85%, 1,2-dilinolein 0.40-0.42%, trilinolein5.96-6.20%, 1-olein-2,3-dilinolein 15.93-16.58%,1-palmitin-2,3-dilinolein 6.43-6.69%, 1,3-diolein-2-linolein16.20-16.87%, 1-palmitin-2-linolein-3-olein 12.57-13.09%,1,3-dipalmitin-2-linolein 2.79-2.91%, triolein 17.69-18.42% and1-palmitin-2,3-diolein 9.87-10.27%.

The above contents refer to the mass percentage contents of diglycerideand triglyceride compounds in the Coix seed oil. 5 diglyceride and 8triglyceride monomer compounds can be separated by using preparativechromatography from the Coix seed oil prepared by the following steps,and their contents can be obtained by weighing and calculating theproducts. They can also be obtained according to conventional analyticmethods in the art.

The Coix seed oil has the following physicochemical constants based onthe fatty oils: specific gravity at 20 0.916-0.920, refractive index at20° C. 1.471-1.474, acid value<0.2, iodine value 100-106, saponificationvalue 186-195.

The Coix seed oil of the invention can be prepared by a refining processof crude Coix seed oil extracted by supercritical carbon dioxideextraction,

wherein:

the process of the supercritical carbon dioxide extraction comprisessteps of:

crushing Coix seeds into 10 mesh-80 mesh powder and extracting sameusing a supercritical CO₂ extraction system in which Coix seed powder isput in 600 L×2 extractors; the CO₂ preheater, extractor and separationcolumn are heated by jacketed circulating hot water to make theextraction temperature and separation temperature to reach 33-45° C. and30-45° C., respectively; the outlet temperatures of separator I andseparator II are kept at 20-50° C. and 15-35° C., respectively; theliquid CO₂ is pressed at a flow rate of 1-3 t/h into the CO₂ preheatervia a high pressure pump, turning into a fluid in supercritical state;in the extractor, an oil is extracted into the CO₂ fluid at a pressureof 19-23 Mpa; then the CO₂ fluid with this oil enters the separationcolumn in which the pressure is controlled to 7-10 Mpa to separate thisoil; the CO₂ gas out from the separation column enters sequentially intoseparator I and separator II in which the pressure is sustained at 5-7Mpa and 4-6 Mpa, respectively; impurities such as water separatedtherefrom are discarded; the CO₂ gas returns to liquid CO₂ for reusethrough a condenser; and a continuous extraction for 2-3 h affords thecrude Coix seed oil; and

the refining process comprises steps of:

adding petroleum ether (bp. 60° C.-90° C.) into the Coix seed oilobtained by the supercritical CO₂ extraction in an amount of 60% of theoil weight; adding 2% NaOH aqueous solution in an amount ranging from36% to 56% of the oil weight according to the acid value; after stirringthe mixture for 10 min and standing for 18-24 h, removing the lowerniger layer; washing the upper layer with purified water and lettingstand for 18-24 h; after the removal of the lower waste water, washingthe upper layer again; after another standing for 40-50 h, removing thelower waste water; and demulsifying the upper layer with acetone in anamount of 70%-90% of the oil weight; after standing for 2-4 h, removingthe lower waste acetone and adding 3% to 8% of activated neutral aluminaby weight of crude oil in the upper oil layer; stirring the mixture for30 min, then filtering off the precipitation; heating the filtrate andadding 2% to 6% of activated kaolin by weight of crude oil; stirring themixture for 30 min at 40-50° C. and then filtering off theprecipitation; concentrating the filtrate under a reduced pressure torecover the solvent, then washing again with purified water; afterstanding for 1-2 h, removing the lower waste water and heating the upperoil layer and vacuum drying it under nitrogen atmosphere; then adding 8to 12% activated neutral alumina by weight of crude oil and stirring themixture and allowing to stand at a cold place; after filtrating,concentrating the filtrated oil via heating under vacuum in a nitrogenatmosphere and sterilizing the oil via dry heat sterilization undervacuum at 160-170° C. for 1-2 h; after cooling, filtering the oilthrough a 0.2 μm microporous membrane; then split charging the obtainedCoix seed oil in 500 mL glass infusion bottles, nitrogenizing andsealing the bottles.

Preferably, the refining process comprises steps of:

adding petroleum ether (bp. 60° C.-90° C.) into the Coix seed oilobtained by the supercritical CO₂ extraction in an amount of 60% of theoil weight; adding 2% NaOH aqueous solution in an amount ranging from36% to 56% of the oil weight according to the acid value; after stirringthe mixture for 10 min and standing for 20 h, removing the lower nigerlayer; washing the upper layer with purified water and letting stand for22 h; after the removal of the lower waste water, washing the upperlayer again; after standing for another 46 h, removing the lower wastewater; demulsifying the upper layer with acetone in an amount of 70%-90%by weight of the crude oil and standing for 3 h; removing the lowerwaste acetone and adding 5% of activated neutral alumina by weight ofcrude oil in the upper oil layer; stirring the mixture for 30 min, thenfiltering off the precipitation; heating the filtrate, and adding 4% ofactivated kaolin by weight of crude oil; stirring the mixture for 30 minat 40-50° C., and then filtering off the precipitation; concentratingthe filtrate under a reduced pressure to recover the solvent, thenwashing again with purified water; after standing for 1 h, removing thelower waste water; heating the upper oil layer and vacuum drying it innitrogen atmosphere; then adding 8%-12% activated neutral alumina,stirring the mixture and allowing to stand at a cold place; afterfiltration, concentrating the filtrated oil via heating under vacuum ina nitrogen atmosphere and sterilizing the concentrated oil via dry heatsterilization under vacuum at 160-170° C. for 2 h; after cooling,filtering the oil through a 0.2 μm microporous membrane; then splitcharging the obtained Coix seed oil in 500 mL glass infusion bottles,nitrogenizing and sealing the bottles.

The Coix seed oil of the invention is a yellowish clear liquid with alight odor and a light taste. It is highly soluble in petroleum ether orchloroform, freely soluble in acetone, slightly soluble in ethanol, butinsoluble in water.

The Coix seed oil prepared based on the above methods was detectedaccording to the method in the appendix of “Pharmacopoeia of thePeople's Republic of China” (2010 edition) Volume I. Physicochemicalconstants thereof are: specific gravity at 20 0.916-0.920, refractiveindex at 20° C. 1.471-1.474, acid value<0.2, iodine value 100-106,saponification value 186-195. The acid value according to thePharmacopoeia refers to the weight of potassium hydroxide (inmilligrams) needed to neutralize free fatty acids contained in 1 gram offats, fatty oils, or other similar substances. In the quality study ofoil products, acid value is an important evaluation. As far as the Coixseed oil of the invention, the acid value is less than 0.2. By theoptimization of the preparation process such as supercritical extractionparameters and the purification process like basification, Coix seed oilwas prepared with the following advantages: on the one hand, it has avery low content of free fatty acid impurities, which means a highproduct quality; on the other hand, it gathers a great amount of activeingredients of diglycerides and triglycerides in high purity, and thetypes of diglycerides and triglyceride ingredients therein aredeterminate, and the contents thereof are stable. In addition, otherphysicochemical constants, such as saponification value, iodine value,etc., measured between batches of samples, had a small range ofvariation. It further illustrates that the Coix seed oil of theinvention has a stable quality and a safer clinical use. The preparationmethod of the invention gives a stable product with a high yield and alow cost. It is suitable for the industrial production in view of thesafety and controllability.

The second aspect of the invention is to provide a pharmaceuticalpreparation containing Coix seed oil, specifically, it comprises atherapeutically effective amount of the Coix seed oil of the inventionand one or more pharmaceutically acceptable carriers.

Pharmaceutically acceptable carriers can be selected from pharmaceuticalconventional dilutions, excipients, fillers, emulsifiers, binders,lubricants, absorption accelerators, surfactants, disintegrants,lubricants and antioxidants, if necessary, flavoring agents, sweeteners,preservative and/or coloring agents

Pharmaceutically acceptable carriers can be selected from one or more inthe group consists of: mannitol, sorbitol, sodium metabisulfite, sodiumbisulfite, sodium thiosulfate, cysteine hydrochloride, thioglycolicacid, methionine, soybean lecithin, vitamin C, vitamin E, EDTA disodium,EDTA calcium sodium, monovalent alkali metal carbonate, acetate,phosphate or its aqueous solution, hydrochloric acid, acetic acid,sulfuric acid, phosphoric acid, amino acids, sodium chloride, potassiumchloride, sodium lactate, ethylparaben solution, benzoic acid, potassiumsorbate, chlorhexidine acetate, xylitol, maltose, glucose, fructose,dextran, glycine, starch, sucrose, lactose, mannitol, silicicderivatives, cellulose and its derivatives, alginates, gelatin,polyvinyl pyrrolidone, glycerin, Tween 80, agar-agar, calcium carbonate,calcium bicarbonate, surfactants, polyethylene glycol, cyclodextrin,β-cyclodextrin, phospholipid material, kaolin, talc, and calciumstearate or magnesium stearate.

Pharmaceutical preparation can be oral solid preparations, oral liquidpreparations or injections.

Preferably, the oral solid preparation is selected from any one ofcapsules, tablets, dripping pills, granules, and concentrated pills; theoral liquid preparation is selected from any one of aqueous or oilysuspensions, solutions, emulsions, syrups or elixirs, and a dry productthat may be reconstructed by water or other suitable carrier before use;and the injection is selected from any one of nano suspensions,liposomes, emulsions, lyophilized powder for injection and aqueousinjection.

More preferably, the injection comprises the following components: theCoix seed oil of the invention 50-350 g, soybean lecithin for injectionor soybean lecithin acceptable for injection 10-40 g, glycerin forinjection or glycerin acceptable for injection 15-50 g, and water forinjection adds to 1000 mL.

The injection of the invention can be prepared by a method comprisingsteps of:

adding appropriate amount of water for injection to a formulated amountof soybean lecithin for injection or soybean lecithin acceptable forinjection; dispersing the mixture with a high shear dispersingemulsifier to give a dispersion without bulks or granules; adding aformulated amount of glycerin for injection or glycerin acceptable forinjection; then adding water for injection to a specified amount, andstirring the mixture to give a water phase;

weighing a formulated amount of Coix seed oil; heating the weighed oiland the water phase separately to 60-70° C., then mixing them andemulsifying the mixture in a high pressure homogenizer, in which the lowpressure is 5-12 MPa and the high pressure is 25-50 MPa; repeating thecycle of homogenization for 3-6 times until the amount of particlesbelow 2 μm is no less than 95% and particles above 5 μm areundetectable; if necessary, using NaOH or HCl to adjust the pH to 4.8 to8.5, preferably 6.8 to 7.0, most preferably 6.8; and

filtering the resulting homogeneous emulsion by nitrogen pressurethrough a microporous filter of 3 μm or less; filling the emulsion,nitrogenizing, sterilizing and cooling to afford the injection.

The capsule of the invention comprises the following components: Coixseed oil 200-800 g, antioxidant(s) and/or emulsifier(s) 0.20-0.60 g for1000 capsules.

The capsule of the invention can be prepared by a method comprisingsteps of:

preparing glue solution: weighing gelatin, purified water, glycerin anda preservative at a weight ratio of 1:0.6-1.2:0.3-0.8:0.0001-0.01;adding glycerin, purified water and preservative (selected from any oneof 10% ethylparaben solution, benoic acid, potassium sorbate andchlorhexidine acetate) sequentially into a glue melting tank; heating to70° C.-90; then adding gelatin and constantly stirring the mixture undervacuum until the gelatin is completely dissolved; filtering the gluesolution and storing the filtered glue solution at 56-62 for use;

preparing drug liquid: adding formulated amount of Coix seed oil,antioxidant (Vitamin E) and/or emulsifier (Tween 80) into an dosingtank, and stirring the mixture constantly until being homogeneouslymixed; and

pressing capsules: choosing proper pellet dies according to the capsulesize; pressing capsules in a tempreture of 15-30 and a relative humidityof less than 35%; drying the pressed and shaped capsules; after removingcapsules of abnormal size, washing the normal capsules with 95%medicinal ethanol, and drying them continuously till the moisturecontent is less than 12%; visually inspecting and removing unqualifiedcapsules; finally printing and packaging to afford the capsules.

It is demonstrated, in pharmacodynamic experiments, that the Coix seedoil of the invention and the pharmaceutical preparation thereof haveshown different degrees of inhibition on a variety of human tumor celllines. Thus, the Coix seed oil of the invention and pharmaceuticalpreparations thereof can be used as therapeutical drugs of neoplasticdiseases.

Therefore, another aspect of the invention is to provide a method of thetreatment of a tumor or an inflammation in a mammal (including human),comprising administering to the mammal (including human) in need atherapeutically effective amount of the Coix seed oil of the inventionor a pharmaceutical preparation thereof.

The Coix seed oil of the invention or the pharmaceutical preparationthereof can be administered alone or in combination with achemotherapeutic drug selected from one or more of platinums, alkylatingagents, difluoro nucleosides, antibiotics, cytotoxics and/or hormons.

Preferably, platinum is selected from cisplatin and carboplatin, thealkylating agent is selected from cyclophosphamide, the difluoronucleoside is selected from gemcitabine hydrochloride, the antibioticsare selected from mitoxantrone, mitomycin, the cytotoxics are selectedfrom docetaxel, paclitaxel, and the hormone is selected from leuprorelinacetate. Said cancer refers to lung cancer, liver cancer, pancreaticcancer, prostate cancer, ovarian cancer, breast cancer, sarcomatoidcarcinoma or cancer sarcoma, in early, middle or late stage; and theinflammation can be prostatic hyperplasia.

The following experimental data are used to illustrate anti-tumor orantiinflammation benefical effects of the Coix seed oil of the inventionand the pharmaceutical preparations thereof.

I. Inhibition of Coix Seed Oil and Preparations Thereof on 8 Human TumorCell Lines in MTT Method In Vitro

A. Experimental Materials and the Preparation Thereof:

-   -   (1) Cell lines: PANC-1 (human pancreatic cancer cells), SKOV3        (human ovarian cancer cells), MCF-7 (human breast cancer cells),        Bcap-37 (human breast cancer cells), SMMC-7721 (human hepatic        cancer cells), HepG-2 (human hepatic cancer cells), A549 (human        lung cancer cells) and H460 (human lung cancer cells), storaged        and passaged maintainably in Research and Evaluation Center for        Pharmacology, Shanghai Institute of Pharmaceutical Industry;    -   (2) DMEM complete medium supplied with 10% newborn calf serum        (GIBCO BRL), 1% of penicillin (100 U/mL)+streptomycin (100        μg/mL);    -   (3) 0.25% trypsin solution, purchased from Invitrogen Corp. and        storaged at −20;    -   (4) Phosphate buffer (PBS): NaCl 8 g, KCl 0.2 g, Na₂HPO₄ 1.15 g        and KH₂PO₄ 0.2 g, dissolved in 1 L double-distilled water and        autoclaved at 121 for 20 min, then storaged at 4;    -   (5) MTT (AMRESCO) solution: 5 mg/ml in PBS;    -   (6) Formazan crystal dissolving solution: SDS 10 g, isobutanol 5        ml and concentrated hydrochloric acid 0.1 ml, dissolved in 100        ml of deionized double distilled water.

B. Experimental Method

The inhibition effects of samples on the above-mentioned cell lines weredetected by using MTT method. The specific procedures were as follows:

(1) Cell culture: (a) Storaged cells were taken out from the liquidnitrogen, thawed quickly in a 37 water bath, then asepticallytransferred into 6 ml of cellular medium in a 10 ml centrifugal tube,centrifuged at 1000 rpm for 5 min. The supernatant was discarded, thenthe precipitated cells were re-suspensed in 5-6 ml cellular media bypipetting and transferred into a flask in a 37 incubator for cellculture; (b) Next day, the flask was taken out from the incubator andthe used medium was discarded, then the cells were incubated in 5-6 mlfresh medium in the 37 incubator; (c) On the third day, the flask wastaken out from the incubator and the used medium was discarded, then 2-3ml of PBS (pH7.4) was added into the flask with rocking for cleaning itand the used PBS was discarded. Such a cellular cleaning step wasrepeated once again. 3-5 drops of 0.25% trypsin solution were added intothe flask with sloshing, thus well-distributed in it. The flask wascapped and placed in a 37 incubator for about 3 min, and the separationof cells from the flask wall was observed under the microscope. 2 ml ofcellular medium was added and cells were separated completely from theflask wall by pipetting, then the cell suspension was transferred into 2separate clean flasks, each containing 5-6 ml medium. The cellsuspension was well-distributed by pipetting, then the flask was placedin a 37 incubator. (d) Step(c) was repeated every other day. In thewhole cultivation process, adherent cells were not allowed to grow toodense and suspension cells were always maintained at a logarithmicgrowth stage.

(2) Preparation of the sample and the control: A proper amount of sampleof Coix seed oil (oil of Job's tears seed) was dissolved in DMSO toobtain a solution in a concentration of 10 mg/ml. This solution wasdiluted in a gradient dilution with PBS to obtain a set of samplesolutions in the concentration of 10 mg/ml, 5000 μg/ml, 2500 μg/ml, 1250μg/ml, 625 μg/ml and 312.5 μg/ml, respectively.

(3) Each diluted sample solution was added into duplicated wells of a 96well flat-bottom microplate (10 μl/well). The correspondingly dilutedDMSO solutions, as controls, were added into the wells of themicroplate.

(4) Cells in a logarithmic growth stage were trypsinized and washed,then re-suspended in the medium containing 10% calf serum. The number ofliving cells was counted in Trypan blue dye exclusion method and cellsuspensions were adjusted into a density of 2×10⁵ cell/ml.

(5) The cell-contained 96 well flat-bottom microplate was placed in a 37incubator and cells were incubated under 5% CO₂ for 48 h.

(6) 20 μl of 5 mg/ml MTT solution was added into each well and cellswere incubated continuously in the incubator for 3-4 h.

(7) 100 μl of crystal dissolving solution was added into each well andcells were incubated continuously in the incubator overnight, so as todissolve the resulted formazan crystals sufficiently. Then, theabsorbance value was measured at 570 nm for each well.

(8) Based on absorbance values, inhibition rates on the cell growth werecalculated for sample groups of various concentrations. The calculationformula was as follows:

(1−mean absorbance of experimental wells/mean absorbance of controlwells)×100%

C. Experimental Results

TABLE 1 Inhibition rates of samples in various concentrations on thecell growth in 8 cell lines (%) Concentration of Sample Cell line 1000μg/ml 500 μg/ml 250 μg/ml 125 μg/ml 62.5 μg/ml 31.25 μg/ml PANC-1 98.7773.83 26.24 18.93 15.96 2.95 SKOV3 98.85 59.52 26.70 16.43 3.43 1.31MCF-7 98.47 65.68 23.29 11.85 7.02 0.42 Bcap-37 99.63 73.03 36.34 17.342.29 1.40 SMMC-7721 98.54 70.73 39.44 22.37 14.70 3.12 HepG-2 98.4765.35 38.30 26.22 16.02 1.07 A549 99.02 74.97 56.85 42.61 22.00 1.48H460 97.16 73.47 56.36 44.13 18.45 7.14

TABLE 2 IC₅₀ values of samples in 8 cell lines in vitro (μg/ml) SamplePositive control Cell line Coix seed oil (Taxol) PANC-1 213.1 0.44 SKOV3262.8 0.22 MCF-7 275.5 0.18 Bcap-37 220.7 0.28 SMMC-7721 205.9 0.41HepG-2 222.5 0.45 A549 173.2 0.46 H460 166.9 0.49

D. Conclusion

The Coix seed oil of the invention in various concentrations has shownthe inhibition on 8 human tumor cell lines in different degrees.

2. Inhibition Rate of the Injection of the Invention on the Growth ofA549 Human Lung Cancer Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), Cisplatin (QiluPhamaceutical Co., Ltd), blank fat emulsion and normal saline

B. Experimental Method

A549 Human lung cancer cells cryopreserved in liquid nitrogen wererecovered, and incubated in a 37 incubator under 5% CO₂. After beingsubcultured, cells in a logarithmic growth stage were distributed withnormal saline to be a cell suspension in a concentration of 1-2×10⁷cells/ml. The cell suspension was inoculated into BALB/C nude mice (SPFgrade, 18-20 g, 6 weeks old, male) subcutaneously at right axillary. Thetumor tissues were aseptically taken from the 2^(nd) generation ofxenograft model of A549 human lung cancer in a vigorous growth stage andcut into small uniform pieces in the size of 1-2 mm³. The right axillaryof each nude mouse was inoculated one piece subcutaneously via a trocar.When the inoculated tumor could be touched, the mice were randomlygrouped and administered according to the experimental design program.All contacted feed, padding, cages and equipment, etc., should beautoclaved prior to use. The nude mice were fed in a laminar flow rack.Tumor sizes and animal weights were observed and tested dynamically.Mice in each group were sacrificed 3 weeks later or so, and tumors weresurgically removed and weighed. The inhibition rate on tumor wascalculated according to the following formula:

Inhibition rate %=[(Mean tumor weight of the control group−mean tumorweight of the treatment group)/mean tumor weight of the controlgroup]×100%;

Q value of antitumor effect in drug combination was calculated accordingto Jin's formula:

Q=E _(a+b)/(E _(a) +E _(b) −E _(a) ×E _(b))

Wherein E_(a+b)=the tumor inhibition rate of drug combination, E_(a) orE_(b)=the tumor inhibition rate of drug A or drug B, respectively. If Qvalue=0.85-1.15, an additive effect (+) was shown; if Q value>1.15, asynergistic effect (++) was shown.

C. Experimental Results

TABLE 3 Efficacy of anti-tumor on Xenograft model of A549 human lungcancer inoculated subcutaneously in nude mice Number Weight of Weight ofDosage of animal animal (g) tumor (g) Inhibition Sample group Dosageregimen start/end start/end x ± SD Rate % Q The injection 25 ml/kg iv ×10qd 6/6 20.2/24.3 0.623 ± 0.19** 52.87 The injection 12.5 ml/kg iv ×10qd 6/6 20.1/25.2 0.788 ± 0.19** 40.39 The injection 6.25 ml/kg iv ×10qd 6/6 20.6/25.3 0.845 ± 0.15** 36.08 The injection + (25 ml + iv ×10qd + 6/6 20.1/25.3 0.458 ± 0.10** 65.36 0.8987 Cisplatin 1 mg)/kg ip ×7qd The injection + (12.5 ml + iv × 10qd + 6/6 20.2/24.5 0.501 ± 0.11**62.10 0.9480 Cisplatin 1 mg)/kg ip × 7qd The injection + (6.25 ml + iv ×10qd + 6/6 20.1/25.4 0.558 ± 0.11** 57.79 0.9172 Cisplatin 1 mg)/kg ip ×7qd Cisplatin 1 mg/kg ip × 7qd 6/6 20.2/24.0 0.765 ± 0.11** 42.13Cisplatin 2 mg/kg ip × 7qd 6/6 20.4/23.1 0.181 ± 0.06** 86.31 Blank fat25 ml/kg iv × 10qd 6/6 20.1/26.5 1.325 ± 0.34  — emulsion Control (NS)25 ml/kg iv × 10qd 6/6 20.3/26.6 1.322 ± 0.32  —  *P < 0.05, **P < 0.01,compared with NS negative control group.

D. Experimental Conclusion

It is shown that the injection of the invention (25 ml/kg, 12.5 ml/kgand 6.25 ml/kg, iv×10 qd) has a significant tumor inhibition effect onthe growth of A549 human lung cancer transplanted in nude mice.

It is shown that the tumor inhibition effect of the co-administration ofthe injection of the invention with cisplatin was significantly strongerthan that of the injection of the invention or that of cisplatin, alone,on A549 human lung cancer transplanted in nude mice, i.e., theco-administration has shown a significant additive effect.

3. The Tumor Inhibition Rate of the Injection of the Invention on theGrowth of QGY Human Hepatoma Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), Adriamycin (Pfizer ItaliaS.r.1), blank fat emulsion and normal saline

B. Experimental Method

QGY human hepatoma cells cryopreserved in liquid nitrogen wererecovered, and incubated in a 37 incubator under 5% CO₂. Other stepswere the same as mentioned in 2-B.

C. Experimental Results

TABLE 4 Efficacy of anti-tumor on Xenograft model of QGY human hepatomainoculated subcutaneously in nude mice Number Weight of Weight of Dosageof animal animal (g) tumor (g) Inhibition Sample group Dosage regimenstart/end start/end x ± SD rate % Q The injection 25 ml/kg iv × 10qd 6/621.4/25.6 0.680 ± 0.18** 41.18 The injection 12.5 ml/kg iv × 10qd 6/621.4/25.2 0.787 ± 0.15** 31.92 The injection 6.25 ml/kg iv × 10qd 6/621.5/24.9 0.790 ± 0.18*  31.66 The injection + (25 ml + Iv × 10qd + 6/621.8/24.9 0.502 ± 0.16** 56.57 0.8870 Adriamycin 1 mg)/kg ip × 7qd Theinjection + (12.5 ml + iv × 10qd + 6/6 21.5/24.7 0.598 ± 0.15** 48.270.8312 Adriamycin 1 mg)/kg ip × 7qd The injection + (6.25 ml + iv ×10qd + 6/6 21.8/24.7 0.627 ± 0.12** 45.76 0.7902 Adriamycin 1 mg)/kg ip× 7qd Adriamycin 1 mg/kg ip × 7qd 6/6 21.3/24.6 0.712 ± 0.18** 38.41Adriamycin 2 mg/kg ip × 7qd 6/6 21.8/23.1 0.338 ± 0.17** 70.76 Blank fat25 ml/kg iv × 10qd 6/6 21.6/26.9 1.125 ± 0.15  — mulsion NS 25 ml/kg iv× 10qd 6/6 21.5/26.7 1.156 ± 0.24  —  *P < 0.05, **P < 0.01, comparedwith negative control group (NS).

D. Experimental Conclusion

It is shown that the injection of the invention (25 ml/kg, 12.5 ml/kgand 6.25 ml/kg, iv×10 qd) has a significant tumor inhibition effect onthe growth of QGY human hepatoma transplanted in nude mice.

It is shown that the tumor inhibition effect of the co-administration ofthe injection of the invention with Adriamycin was significantlystronger than that of the injection of the invention or Adriamycin,alone, on QGY human hepatoma transplanted in nude mice.

4. The Tumor Inhibition Rate of the Injection of the Invention on LM-3Human Hepatoma Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), cyclophosphamide (JiangsuHengrui Medicine Co., Ltd), blank fat emulsion and normal saline

B. Experimental Method

LM-3 human hepatoma cells cryopreserved in liquid nitrogen wererecovered, and incubated in a 37 incubator under 5% CO₂. Other stepswere the same as mentioned in 2-B.

C. Experimental Results

TABLE 5 Efficacy of anti-tumor on Xenograft model of LM-3 human hepatomainoculated subcutaneously in nude mice Number Weight of Weight of Dosageof animal animal (g) tumor (g) Inhibition Sample group Dosage regimenstart/end start/end x ± SD rate % Q The injection 25 ml/kg iv × 10qd 6/620.2/26.3 1.043 ± 0.16** 32.05 The injection 12.5 ml/kg iv × 10qd 6/620.3/26.2 1.092 ± 0.28** 28.86 The injection 6.25 ml/kg iv × 10qd 6/620.4/27.0 1.251 ± 0.27** 18.50 The injection + (25 ml + iv × 10qd + 6/620.4/26.6 0.755 ± 0.13** 50.81 0.9972 cyclophosphamide 15 mg)/kg ip ×7qd The injection + (12.5 ml + iv × 10qd + 6/6 20.3/28.1 0.778 ± 0.17**49.32 1.0137 cyclophosphamide 15 mg)/kg ip × 7qd The injection + (6.25ml + iv × 10qd + 6/6 20.4/25.8 0.862 ± 0.19** 43.84 1.0648cyclophosphamide 15 mg)/kg ip × 7qd cyclophosphamide 15 mg/kg ip × 7qd6/6 20.7/24.8 1.108 ± 0.09** 27.82 cyclophosphamide 30 mg/kg ip × 7qd6/6 20.5/25.0 0.265 ± 0.12** 82.74 Blank fat emulsion 25 ml/kg iv × 10qd6/6 20.4/27.8 1.557 ± 0.30  — NS 25 ml/kg iv × 10qd 6/6 20.6/28.0 1.535± 0.28  —  *P < 0.05, **P < 0.01, compared with negative control group(NS).

D. Experimental Conclusion

It is shown that the injection of the invention (25 ml/kg, 12.5 ml/kgand 6.25 ml/kg, iv×10 qd) has a significant tumor inhibition effect onthe growth of LM-3 human hepatoma transplanted in nude mice.

It is shown that the tumor inhibition effect of the co-administration ofthe injection of the invention with cyclophosphamide was significantlystronger than that of the injection of the invention orcyclophosphamide, alone, on LM-3 human hepatoma transplanted in nudemice, i.e., the co-administration has shown a significant additiveeffect.

5. The Tumor Inhibition Rate of the Injection of the Invention on theGrowth of SMMC-7721 Human Hepatoma Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), gemcitabine hydrochloride(LILLY FRANCE), blank fat emulsion and normal saline

B. Experimental Method

SMMC-7721 human hepatoma cells cryopreserved in liquid nitrogen wererecovered, and incubated in a 37 incubator under 5% CO₂. Other stepswere the same as mentioned in 2-B.

C. Experimental Results

TABLE 6 Efficacy of anti-tumor on Xenograft model of SMMC-7721 humanhepatoma inoculated subcutaneously in nude mice Number Weight of Weightof Dosage of animal animal (g) tumor (g) Inhibition Sample group Dosageregimen start/end start/end x ± SD rate % Q The injection 25 ml/kg iv ×10qd 6/6 20.4/25.1 0.876 ± 0.13** 49.71 The injection 12.5 ml/kg iv ×10qd 6/6 20.4/25.6 0.942 ± 0.21** 45.92 The injection 6.25 ml/kg iv ×10qd 6/6 20.1/24.8 1.041 ± 0.23** 40.24 The injection + (25 ml + iv ×10qd + 6/6 20.5/26.1 0.610 ± 0.09** 64.98 0.8731 Gemcitabine 25 mg)/kgiv × 3q3d The injection + (12.5 ml + iv × 10qd + 6/6 20.9/26.5 0.632 ±0.14** 63.72 0.8790 Gemcitabine 25 mg)/kg iv × 3q3d The injection +(6.25 ml + iv × 10qd + 6/6 20.4/26.4 0.661 ± 0.16** 62.06 0.8916Gemcitabine 25 mg)/kg iv × 3q3d Gemcitabine 25 mg/kg iv × 3q3d 6/620.1/25.1 0.886 ± 0.24** 49.14 Gemcitabine 50 mg/kg iv × 3q3d 6/620.4/24.2 0.215 ± 0.11** 87.66 Blank fat 25 ml/kg Iv × 10qd 6/620.2/26.1 1.765 ± 0.19  emulsion NS 25 ml/kg iv × 10qd 6/6 20.8/26.91.742 ± 0.24   *P < 0.05, **P < 0.01, compared with negative controlgroup (NS).

D. Experimental Conclusion

It is shown that the injection of the invention (25 ml/kg, 12.5 ml/kgand 6.25 ml/kg, iv×10 qd) has a significant tumor inhibition effect onthe growth of SMMC-7721 human hepatoma transplanted in nude mice.

It is shown that the tumor inhibition effect of the co-administration ofthe injection of the invention with gemcitabine hydrochloride wassignificantly stronger than that of the injection of the invention orgemcitabine hydrochloride, alone, on SMMC-7721 human hepatomatransplanted in nude mice, i.e., the co-administration has shown asignificant additive effect.

6. The Inhibition Rate of the Injection of the Invention on 5180 SarcomaTransplanted in Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), Cyclophosphamide (CTX),Mitoxantrone (DHAD), Mitomycin (MMC) and normal saline

S180 sarcoma: inoculated in Kunmin mice, 19-21 g, female

B. Experimental Method

Ascitic fluid of mice having well-grown 5180 sarcoma was diluted withnormal saline (1:4) to obtain a cell suspension of about 1-2×10⁷cells/ml. Each mouse was inoculated with 0.2 ml of the suspensionsubcutaneously at the right axilla, and the mice were grouped randomly.The administration began from the next day according to the dosageprogram in Table 7. On the tenth day after the inoculation of S180,animals were sacrificed via cervical dislocation and dissected to takeout the tumor block. Weights of tumor were compared and inhibition rateswere calculated for each group.

C. Experimental Results

TABLE 7 Efficacy of anti-tumor on S180 sarcoma model in mice NumberWeight of Dosage of animal tumor (g) Inhibition Sample group Dosageregimen start/end x ± SD rate % Q The injection 25 ml/kg iv × 7 10/101.18 ± 0.23 25.32*  The injection + 25 ml/kg + iv × 7 + 10/10 0.79 ±0.14 50.00** 0.8847 CTX 30 mg/kg ip × 2(1, 3) CTX 30 mg/kg Ip × 2(1, 3)10/10 0.92 ± 0.21 41.77*  The injection + 25 ml/kg + iv × 7 + 10/10 0.52± 0.13 67.09** 1.0627 DHAD 2 mg/kg ip × 2(1, 3) DHAD 2 mg/kg Ip × 2(1,3) 10/10 0.78 ± 0.20 50.63** The injection + 25 ml/kg + iv × 7 + 10/100.62 ± 0.12 60.76** 1.1220 MMC 1.5 mg/kg ip × 2(1, 3) MMC 1.5 mg/kg Ip ×2(1, 3) 10/10 0.97 ± 0.19 38.61*  Control (NS) 25 ml/kg iv × 7 10/101.58 ± 0.36 —  *P < 0.05, **P < 0.01, compared with negative controlgroup (NS).

D. Experimental Conclusion

It is shown in the results that the injection of the invention incombination with CTX, DHAD or MMC has a tumor inhibition effect that issignificantly stronger than that of the injection of the invention orchemotherapy drug alone, i.e., the co-administration has shown asignificant additive effect.

7. The Inhibition Rate of the Injection of the Invention on W256 CancerSarcoma Transplanted in Rats

A. Experimental Materials

The injection of the invention (10 g/100 ml), Cyclophosphamide (CTX) andnormal saline

W256 cancer sarcoma: inoculated in Wistar rats, 50-60 g, female

B. Experimental Method

Ascitic fluid of rats with well-grown W256 cancer sarcoma was dilutedwith normal saline to obtain a cell suspension of about 1-2×10⁷cells/ml. Each rat was inoculated with 0.2 ml of the suspension at theright axilla subcutaneously. The rats were grouped randomly next day andbegan administration. Two weeks later, animals were sacrificed anddissected to take out the tumor block. Weights of tumor were comparedbetween each experimental group and the control group and tumorinhibition rates were calculated.

C. Experimental Results

TABLE 8 Efficacy of anti-tumor on the model of W256 cancer sarcoma inrats Number Weight of Sample Dosage of animal tumor (g) Inhibition groupDosage regimen start/end x ± SD rate % Q CTX 30 mg/kg iv × 7 10/8  0.42± 0.85 87.68** CTX 10 mg/kg iv × 2(3, 5) 10/10 2.12 ± 1.22 37.83*  Theinjection 20 ml/kg iv × 10 10/10 1.44 ± 0.72 57.78** The injection + 20ml/kg + iv × 10 + 10/10 0.94 ± 0.32 72.43** 0.9821 CTX 10 mg/kg iv ×2(3, 5) The injection 10 ml/kg iv × 10 10/10 1.88 ± 0.71 44.87** Theinjection + 10 ml/kg + iv × 10 + 10/10 1.02 ± 0.58 70.09** 1.0664 CTX 10mg/kg iv × 2(3, 5) The injection 5 ml/kg iv × 10 10/9  2.13 ± 1.1937.54*  The injection + 5 ml/kg + iv × 10 + 10/10 1.68 ± 0.98 50.73**0.8293 CTX 10 mg/kg iv × 2(3, 5) Control (NS) 20 ml/kg iv × 10 10/103.41 ± 1.16 —  *P < 0.05, **P < 0.01, compared with negative controlgroup (NS).

D. Experimental Conclusion

It is shown in the results that the injection of the invention incombination with CTX has a tumor inhibition effect that is significantlystronger than that of the injection of the invention or the chemotherapydrug alone, i.e., the co-administration has shown a significant additiveeffect.

8. The Tumor Inhibition Rate of the Injection of the Invention on PANC-1Human Pancreatic Cancer Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), Gemcitabine hydrochlorideand normal saline

PANC-1 human pancreatic cancer cells: inoculated in BALB/C nude mice,16-18 g, female

B. Experimental Method

Tumor blocks of well-grown PANC-1 human pancreatic cancer wereinoculated subcutaneously in 4-week-old female BALB/C nude mice. Thenude mice were grouped randomly and administered. One week after the endof the administration, the mice were sacrificed and dissected. Tumorblocks were taken out and weighed. Differences of tumor weight betweeneach experimental group and the control group were compared and thetumor inhibition rates were calculated.

C. Experimental Results

TABLE 9 Efficacy of anti-tumor on model of PANC-1 human pancreaticcancer in nude mice Number Weight of Dosage of animal tumor (g)Inhibition Sample group Dosage regimen start/end x ± SD rate % QGemcitabine 60 mg/kg iv × 1 6/6 0.1443 ± 0.081 24.61 The injection 50ml/kg iv × 10 6/6 0.1491 ± 0.013 22.10 The injection + 50 ml/kg + iv ×10 + 6/6 0.0921 ± 0.043 51.88 1.2570 Gemcitabine 60 mg/kg iv × 1 Control(NS) 50 ml/kg iv × 10 6/6 0.1914 ± 0.087 —

It is shown in the results that the injection of the invention incombination with gemcitabine hydrochloride has a tumor inhibition effectthat is significantly stronger than that of the injection of theinvention or the chemotherapy drug alone, i.e., the co-administrationhas shown a significant additive effect.

9. The Tumor Inhibition Rate of the Injection of the Invention on FC-3MHuman Prostate Cancer Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), Leuprolide acetate(Lupron) and normal saline

FC-3M human prostate cancer: inoculated in BALB/C nude mice, 17-21 g,male

B. Experimental Method

Tumor blocks of well-grown FC-3M human prostate cancer were homogenizedin normal saline (1:4). Each nude mouse was inoculated with 0.2 ml ofthe suspension at the right axilla subcutaneously, and the mice weregrouped randomly. The administration began from the next day. 21 Daysafter the inoculation, the mice were sacrificed. Tumor blocks were takenout and weighed. Differences of tumor weight between each experimentalgroup and the control group were compared and the tumor inhibition rateswere calculated.

C. Experimental Results

TABLE 10 Efficacy of anti-tumor on model of FC-3M human prostate cancerin nude mice Number Weight of Dosage of animal tumor (g) InhibitionSample group Dosage regimen start/end x ± SD rate % Q Lupron 0.75 ml/kgiv × 1 6/6 0.98 ± 0.23 36.36** Lupron 1.5 ml/kg iv × 1 6/6 0.76 ± 0.1450.65** The injection 12.5 ml/kg iv × 10 6/6 1.12 ± 0.24 27.27  Theinjection + 12.5 ml/kg + iv × 10 + 6/6 0.52 ± 0.13 66.23** 1.2330 Lupron0.75 ml/kg iv × 1 The injection 25 ml/kg iv × 10 6/6 0.77 ± 0.15 50.00**The injection + 25 ml/kg + iv × 10 + 6/6 0.45 ± 0.16 70.78** 0.9397Lupron 1.5 ml/kg iv × 1 Control (NS) 25 ml/kg iv × 10 6/6 1.54 ± 0.20 — *P < 0.05, **P < 0.01, compared with the control group.

It is shown in the results that the injection of the invention incombination with Lupron has a tumor inhibition effect that issignificantly stronger than that of the injection of the invention orthe chemotherapy drug alone, i.e., the co-administration has shown asignificant additive effect.

10. The Tumor Inhibition Rate of the Injection of the Invention onBcap37 Human Breast Cancer Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), Docetaxel injection(Taxotere, 20 mg/vial) and normal saline

Bcap-37 human breast cancer: inoculated in BALB/C nude mice (SPF grade),18-20 g, female

B. Experimental Method

Tumor blocks of well-grown Bcap-37 human breast cancer were homogenizedin normal saline to obtain cell suspensions in the concentration of1-2×10⁷ cells/ml. Each nude mouse was inoculated with 0.2 ml of thesuspension at the right axilla subcutaneously, and the mice were groupedrandomly. The administration began from the 7th day after theinoculation. 20 Days after the inoculation, the mice were sacrificed.Tumor blocks were taken out and weighed. Differences of tumor weightbetween each experimental group and the control group were compared, andtumor inhibition rates and Q values were calculated.

C. Experimental Results

TABLE 11 Efficacy of anti-tumor on model of Bcap-37 human breast cancerin nude mice Number Weight of Dosage of animal tumor (g) InhibitionSample group Dosage regimen start/end x ± SD rate % Q Control (NS) 25ml/kg iv × 10 10/10 2.94 ± 0.81  Docetaxel 25 mg/kg ip × 1 6/6 1.27 ±0.88** 56.80 6.25 ml/kg iv × 10 6/6 1.86 ± 0.32** 36.73 The injection12.5 ml/kg iv × 10 6/6 1.41 ± 0.65** 52.04 25 ml/kg iv × 10 6/6 1.18 ±0.59** 59.86 The injection + 6.25 ml/kg + iv × 10 + 6/6 0.87 ± 0.45**70.41 0.9689 Docetaxel 25 mg/kg ip × 1 The injection + 12.5 ml/kg + iv ×10 + 6/6 0.94 ± 0.98** 68.03 0.8581 Docetaxel 25 mg/kg ip × 1 Theinjection + 25 ml/kg + iv × 10 + 6/6 0.72 ± 0.36** 75.51 0.9135Docetaxel 25 mg/kg ip × 1  *P < 0.05, **P < 0.01, compared with thenegative control group.

The tumor inhibition effects of the injection of the invention incombination with Docetaxel injection were significantly stronger thanthat of the injection of the invention or Docetaxel, alone, on Bcap-37human breast cancer transplanted in nude mice. It has been demonstratedin this experiment that the combination of the injection of theinvention with Docetaxel has shown a significant additive effect.

11. The Tumor Inhibition Rate of the Injection of the Invention onMDA-MB-435 Human Breast Cancer Transplanted in Nude Mice

A. Experimental Materials

The injection of the invention (10 g/100 ml), Paclitaxel injection (30mg/5 ml) and normal saline

MDA-MB-435 human breast cancer: inoculated in BALB/C nude mice (SPFgrade), 18-20 g, female

B. Experimental Method

Tumor blocks of well-grown MDA-MB-435 cancer were homogenized in normalsaline to obtain cell suspensions in the concentration of 1-2×10⁷cells/ml. Each nude mouse was inoculated with 0.2 ml of the suspensionat the right axilla subcutaneously, and the mice were grouped randomly.The administration began from the 7th day after the inoculation. 18 Daysafter the inoculation, the mice were sacrificed. Tumor blocks were takenout and weighed. Differences of tumor weight between each experimentalgroup and the control group were compared, and tumor inhibition ratesand Q values were calculated.

C. Experimental Results

TABLE 12 Efficacy of anti-tumor on the model of MDA-MB-435 human breastcancer in nude mice Number Weight of Dosage of animal tumor (g)Inhibition Sample Dosage regimen start/end x ± SD rate % Q Control (NS)25 ml/kg iv × 10 12/12 2.72 ± 0.39  Paclitaxel 50 mg/kg ip × 1 6/6 1.01± 0.33** 62.87 The injection 6.25 ml/kg iv × 10 6/6 1.53 ± 0.26** 43.7512.5 ml/kg iv × 10 6/6 1.22 ± 0.25** 55.15 25 ml/kg iv × 10 6/6 0.66 ±0.07** 75.74 The injection + 6.25 ml/kg + iv × 10 + 6/6 0.93 ± 0.48**65.81 0.8318 Paclitaxel 50 mg/kg ip × 1 The injection + 12.5 ml/kg + iv× 10 + 6/6 0.76 ± 0.45** 72.06 0.8646 Paclitaxel 50 mg/kg ip × 1 Theinjection + 25 ml/kg + iv × 10 + 6/6 0.48 ± 0.32** 82.35 0.9050Paclitaxel 50 mg/kg ip × 1  *P < 0.05, **P < 0.01, compared with thenegative control group.

The tumor inhibition effects of the injection of the invention incombination with Paclitaxel injection were significantly stronger thanthat of the injection of the invention or Paclitaxel injection, alone,on MDA-MB-435 human breast cancer transplanted in nude mice. It has beendemonstrated in this experiment that the combination of the injection ofthe invention with Paclitaxel injection has shown a significant additiveeffect.

12. The Clinical Efficacy of the Capsules of the Invention inCombination with Chemotherapy in the Treatment of Ovarian Cancer

A. Clinical Data and Treatment Method

58 patients suffering from late stage ovarian cancer were randomlydivided into two groups: Group I, capsule of the invention and Group II,control. 30 Cases in Group I were treated with TC program(Taxol+Carboplatin) in combination with the capsule of the invention;and 28 cases of the control group were treated with TC program merely.The baseline data of both groups were balanced (p>0.05). Taxol (175mg/m²) was administered on day 1 and Carboplatin AUC=5 was administeredon day 1 and repeated every 3 weeks. 4-6 Cycles of chemotherapy wasperformed based on patient's benefit situation. The capsule of theinvention (6 capsules, t.i.d.) was administered continuously until theoccurrence of disease progression or intolerable toxic and side effects.

B. Therapeutic Evaluation

(1) Response rate and disease control rate: Short-term effects wereevaluated and analyzed every 2 cycles of chemotherapy using RECISTcriteria, and the results of evaluation were divided into 4 grades:complete response (CR), partial response (PR), stable disease (SD) andprogression of disease (PD). Response rate (RR) was calculated by usingCR+PR; and disease control rate (DCR) was calculated by using CR+PR+SD.Therapeutical effects were confirmed for all CR and PR patients fourweeks later.

(2) Toxic and side effects: Common Terminology Criteria (NCI-CTC AE)version 3.0 was used to analyze adverse effects. Toxic reactions werescored as 0-V grades.

C. Results

(1) Short-term effects: Group I, the response rate was 46.7% (0 case inCR grade, 14 cases in PR grade, 8 cases in SD grade and 8 cases in PDgrade), and the disease control rate was 73.3%; Group II, the responserate was 39.3% (0 case in CR grade, 11 cases in PR grade, 6 cases in SDgrade and 11 cases in PD grade), and the disease control rate was 60.7%.Both the response rate and the disease control rate in Group I weresignificantly higher than those in Group II.

(2) Toxic and side effects: It has been shown in the evaluation for 58pateints that the most common toxic and side effects weregastrointestinal reactions, myelosuppression, neurotoxicity, myalgia andarthralgia, etc. The incidences of grade ¾ myelosuppression in the groupof the invention (Group I) and the control group (Group II) were 42.5%and 54%, respectively; the incidences of grade ¾ febrileneutropenia/infection in the group of the invention (Group I) and thecontrol group (Group II) were 12% and 19%, respectively. There was nodeath case in both groups. Nausea, vomiting, neurotoxicity, myalgia andarthralgia were more common in the control group. The incidence offatigue in the group of the invention was significantly lower than thatin the control group.

Thus it can be seen that the combination of the capsule of the inventionwith Taxol+Carboplatin has a higher response rate in the treatment oflate stage ovarian cancer and a lower chemotherapy-related toxicreaction, and can effectively improve the patients' life quality.

13. The Effect of the Capsule of the Invention on Prostate HyperplasiaInduced by the Implantation of Urogenital Sinus in Mice

A. Experimental Materials

The capsule of the invention, Prostate Tablet (Cerniton®, PuShiTaiPian)(pollen extracts P5 70 mg, EA10 4 mg), NaCl injection (2.25 g/250 ml;250 ml/bottle), Pentobarbital sodium, olive oil, penicillin sodium forinjection (800,000 units/vial)

Animal: ICR mice, clean grade, fasting overnight prior to use.

B. Experimental Method

Preparation of urogenital sinus: ICR mice in sexual maturity, twentyfemale and ten male, 25-30 g, were caged in the ratio offemale:male=2:1. Every morning, the vulva of each mouse was expanded byusing a pincet to inspect whether a vaginal plug appeared. Thephenomenon of the appearance of a white emboli pluged in the vagina,which was formed by the solidified semen, indicates that the copulationhad occurred. The day when vaginal plug appeared was considered as day 1of pregnancy. The female mouse, at the 16th days after fertilization,was sacrificed and the 16 day-old embryo was aseptically taken out. Theurogenital sinus was placed into a glass petri dish filled with normalsaline and reserved.

Modeling: Sixty male ICR mice (25-30 g) in sexual maturity wereanesthetized with pentobarbital sodium via intraperitoneal injection.The abdoman of each mouse was aseptically incised and the ventralprostate was separated carefully. Urogenital sinus tissues from three 16days-old fetus of the same strain mice were transplanted into theventral prostate. 10 mice in the sham operation group were merelypunctured in abdomen but not implanted with urogenital sinus tissues.All mice were given penicillin (20,000 units, i.p.) for three days afterthe operation.

Grouping and administering: The mice without abnormalities, three daysafter the operation, were divided into the following groups: shamoperation group, model group, positive control group (Cernilton 60mg/kg), and groups of the capsule of the invention (high-dose 9 g/kg,middle-dose 3 g/kg and low-dose 1 g/kg). The intragastric administration(0.1 ml/10 g) was performed for 28 days, and the mice in the shamoperation group and the model group were merely given with the samevolume of olive oil.

Detection indexes: (1) body weight, (2) wet weight of ventral prostateand prostate index (wet weight of prostate/body weight), (3)pathological changes of ventral prostate tissues (Scoring criteria isshown in Table 13).

TABLE 13 Scoring criteria of prostate tissue hyperplasia in pathologicaltest (1) Based on the size of prostatic acinar lumen and the amount ofsecretions in lumen −: Prostatic acinar lumen, in different sizes andirregular forms, with pink-dyed secretions; +: Part of prostatic acinarlumen, dilating and tightly stacking, with increasing and deepeningsecretions; ++: Prostatic acinar lumen, obviously dilating and tightlystacking, with obviously increasing and deepening secretions; (2) Basedon the degree of proliferation of fibroblast in mesenchyme of prostate−: No or occasional hyperplastic fibroblast in mesenchyme; +: A fewhyperplastic fibroblasts in mesenchyme, in scattered distribution; ++:Many hyperplastic fibroblasts in mesenchyme; +++: Extensive hyperplasticfibroblasts in mesenchyme, in clusters or bundles. (3) Based onmorphology, arrangement regularity and hyperplasia degree of glandularepithelia −: Columnar or cuboidal monolayer glandular epithelia,protruding towards acinar lumen to form plicae, with cell nucleilocating at the fundus, and arranging regularly; +: Basically normalepithelial morphology, but less regularly arranged; ++: Part of theepithelia proliferated from monolayer into bilayer, irregularlyarranged; +++: Many epithelia proliferated from monolayer into bilayer,or even 3-4 layers, obviously irregularly arranged.

C. Experimental Results

TABLE 14 Effects on ventral prostate in the model of prostatichyperplasis induced by the implantation of urogenital sinus in mice ( x± s, n = 10) Wet weight Ventral prostate of ventral index (mg/10 g GroupDosage prostate (mg) body weight) Sham operation — 17.8 ± 7.0  4.16 ±1.60 Model — 50.1 ± 14.0^(###) 12.18 ± 3.74^(###) Cernilton 60 mg/kg30.7 ± 9.0**  7.30 ± 2.11** Capsule of the invention  9 g/kg 30.9 ±14.2*  7.25 ± 3.25* Capsule of the invention  3 g/kg 32.3 ± 6.2**  7.47± 2.42** Capsule of the invention  1 g/kg 36.5 ± 8.8*  8.50 ± 2.08*^(###)P < 0.001 vs Sham operation group; *P < 0.05, **P < 0.01 vs Modelgroup, t-test.

TABLE 15 Effects on prostatic acinar lumen and secretions in lumen ofventral prostate in the model of prostatic hyperplasis induced by theimplantation of urogenital sinus in mice (n = 10) Size of prostaticacinar lumen and secretions in lumen* Group Dosage − + ++ Sham operation— 10 0 0 Model — 1 5 4 Cernilton 60 mg/kg 4 5 1 Capsule of the invention 9 g/kg 4 5 0 Capsule of the invention  3 g/kg 3 4 2 Capsule of theinvention  1 g/kg 2 3 2 *The number of animals in corresponding grade ofpathological changes.

TABLE 16 Effect on proliferation of fibroblast in mesenchyme of ventralprostate in the model of prostatic hyperplasis induced by theimplantation of urogenital sinus in mice (n = 10) Degree ofproliferation of fibroblast in mesenchyme of prostate* Group Dosage − +++ +++ Sham operation — 5 4 1 0 model — 0 5 3 2 Cernilton 60 mg/kg 2 8 00 Capsule of the invention  9 g/kg 8 4 0 0 Capsule of the invention  3g/kg 2 6 0 0 Capsule of the invention  1 g/kg 2 6 1 2 *The number ofanimals in corresponding grade of pathological changes.

TABLE 17 Effects on morphology and arrangement of glandular epithelia ofventral prostate in the model of prostatic hyperplasis induced by theimplantation of urogenital sinus in mice (n = 10) Morphology,arrangement regularity and degree of proliferation of glandularepithelia* Group Dosage − + ++ +++ Sham operation — 6 3 1 0 Model — 0 16 3 Cernilton 60 mg/kg 2 6 2 0 Capsule of the invention  9 g/kg 4 3 3 0Capsule of the invention  3 g/kg 0 8 4 0 Capsule of the invention  1g/kg 0 0 9 2 *The number of animals in corresponding grade ofpathological changes.

TABLE 18 Effects on area of prostatic acinar lumen and height ofglandular epithelia of ventral prostate in the model of prostatichyperplasis induced by the implantation of urogenital sinus in mice ( x± s, n = 10) Area of prostatic acinar Height of glandular Group Dosagelumen (×10⁴ μm²) epithelia (μm) Sham operation — 2.53 ± 1.11 11.70 ±3.17 Model — 6.46 ± 3.40^(##) 22.44 ± 4.46^(###) Cernilton 60 mg/kg 2.72± 0.92** 15.19 ± 4.18*** Capsule of the  9 g/kg 2.12 ± 1.11** 13.25 ±3.24*** invention Capsule of the  3 g/kg 2.29 ± 1.04** 15.12 ± 3.32***invention Capsule of the  1 g/kg 3.63 ± 1.26* 18.52 ± 5.22*** invention^(##)P < 0.01, ^(###)P < 0.001 vs Sham operation group; *P < 0.05, **P <0.01, ***P < 0.001 vs Model group, t-test.

In mice with prostatic hyperplasis induced by the implantation ofurogenital sinus, the intragastric administration of 1-9 g/kg of thecapsule of the invention for 28 days has significantly reduced theelevation of wet weight of ventral prostate and prostate index;obviously improved pathological changes, e.g., the proliferation offibroblast in mesenchyme of prostate, the amplity of prostatic acinarlumen, the proliferation of glandular epithelia and the increase ofsecretions in lumen, etc.; and significantly decreased areas ofprostatic acinar lumen and heights of glandular epithelia. It is clearthat the capsule of the invention has significant therapeutical effectson the prostatic hyperplasis induced by the implantation of urogenitalsinus in mice.

Summing up the above, the Coix seed oil of the invention has certaininhibition effects on the growth of A549 human lung cancer, QGY, LM-3and SMMC-7721 human hepatic cancers, S180 sarcoma, W256 cancer sarcoma,etc., transplanted in nude mice. The combination of the injection or thecapsule of the invention with small doses of cisplatin,cyclophosphamide, gemcitabine hydrochloride, mitoxantrone, mitomycin,Lupron, docetaxel, paclitaxel (Taxol) or carboplatin has a significantadditive effect on the growth of A549 human lung cancer, LM-3 andSMMC-7721 human hepatic cancers, S180 sarcoma, W256 cancer sarcoma,human pancreatic cancer, prostate cancer, breast cancer and ovariancancer. The capsule of the invention has significant therapeutic effecton prostatic hyperplasia in mice.

Further experiments have confirmed that the Coix seed oil of theinvention and preparations thereof can achieve the desired effectsdescribed in the above experimental examples.

The following examples further illustrate the invention, but are notconstrued as a limitation of the invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Example 1 Preparationof Coix Seed Oil

Supercritical carbon dioxide extraction: Coix seeds were crushed into 60mesh powder and extracted using 600 L×2 supercritical CO₂ extractors.Coix seed powder was put in an extractor. The CO₂ preheater, extractorand separation column were heated by jacketed circulating hot water, sothat the extraction temperature and separation temperature reached 40and 45, respectively, and the outlet temperatures of separator I andseparator II were kept 50 and 35, respectively. Liquid CO₂ was pressedinto the CO₂ preheater via a high pressure pump at a flow rate of 2 t/h,turning into a fluid in supercritical state. In the extractor, an oilwas extracted into the CO₂ fluid at a pressure of 20 Mpa. Then the CO₂fluid with this oil entered a separation column, and the pressure of theseparation column was controlled to 7 Mpa to separate the oil. The CO₂gas out from the separation column entered sequentially into separator Iand separator II, in which the pressures were sustained at 7 Mpa and 6Mpa, respectively. Impurities like water separated therefrom werediscarded. The CO₂ gas returned to liquid CO₂ for reuse through acondenser. A continuous extraction for 2.5 h afforded a crude Coix seedoil.

Refining: To the crude Coix seed oil obtained by supercritical CO₂extraction was added petroleum ether (60) of 60% of the oil weight. 2%NaOH aqueous solution of 45% of the oil weight was added according tothe acid value. After stirring for 10 min, then standing for 20 h, thelower niger layer was removed. The upper layer was washed with purifiedwater and let stand for 22 h. After the removal of the lower wastewater, the upper layer went on a second washing. After another standingfor 46 h, the lower waste water was removed, and the upper layer wasdemulsified by adding acetone of 80% of the oil weight. After standingfor 3 h, the lower waste acetone was removed. The upper oil layer wasadded 5% of activated neutral alumina by weight of crude oil, stirredfor 30 min, and filtered. The filtrate was heated, added with 4% ofactivated kaolin by weight of the crude oil, stirred for 30 min at 45,and then filtered. The filtrate was concentrated under a reducedpressure to recover the solvent, and washed again with purified water.After standing for 1 h, the lower waste water was removed. The upper oilwas heated and vacuum dried under a nitrogen atmosphere. Then activatedneutral alumina (10% of the oil weight) was added. The mixture wasstirred, and allowed to stand at a cold place. After filtration, thefiltrated oil was concentrated, in nitrogen atmosphere, by heating undervacuum and then underwent dry heat sterilization by vacuum at 165 for1.5 h. After cooling, the oil was filtered through a 0.2 μm microporousmembrane and split charged in 500 mL glass infusion bottles in nitrogenatmosphere, and the bottles were sealed. The Coix seed oil was thusobtained in a yield of 4.5%. Physicochemical constants were detected as:specific gravity at 20, 0.915; refractive index at 20, 1.471; acid value0.18; iodine value 102; and saponification value 190.

Example 2 Preparation of Coix Seed Oil

Supercritical carbon dioxide extraction: Coix seeds were crushed into 80mesh powder and extracted using 600 L×2 supercritical CO₂ extractors.Coix seed powder was put in an extractor. The CO₂ preheater, extractorand separation column were heated by jacketed circulating hot water, sothat the extraction temperature and separation temperature reached 40and 40, respectively, and the outlet temperatures of separator I andseparator II were kept 20 and 15, respectively. Liquid CO₂ was pressedinto the CO₂ preheater via a high pressure pump at a flow rate of 1 t/h,turning into a fluid in supercritical state. In the extractor, an oilwas extracted into the CO₂ fluid at a pressure of 22 Mpa. Then the CO₂fluid with this oil entered a separation column, and the pressure of theseparation column was controlled to 8 Mpa to separate the oil. The CO₂gas out from the separation column entered sequentially into separator Iand separator II, in which the pressures were sustained at 6 Mpa and 5Mpa, respectively. Impurities like water separated therefrom werediscarded. The CO₂ gas returned to liquid CO₂ for reuse through acondenser. A continuous extraction for 2 h afforded a crude Coix seedoil.

Refining: To the crude Coix seed oil obtained by supercritical CO₂extraction was added petroleum ether (90) of 60% of the oil weight. 2%NaOH aqueous solution of 56% of the oil weight was added according tothe acid value. After stirring for 10 min, then standing for 22 h, thelower niger layer was removed. The upper layer was washed with purifiedwater and let stand for 20 h. After the removal of the lower wastewater, the upper layer went on a second washing. After another standingfor 48 h, the lower waste water was removed, and the upper layer wasdemulsified by adding acetone of 90% of the oil weight. After standingfor 2 h, the lower waste acetone was removed. The upper oil layer wasadded 8% of activated neutral alumina by weight of crude oil, stirredfor 30 min, and filtered. The filtrate was heated, added with 6% ofactivated kaolin by weight of the crude oil, stirred for 30 min at 42,and then filtered. The filtrate was concentrated under a reducedpressure to recover the solvent, and washed again with purified water.After standing for 2 h, the lower waste water was removed. The upper oilwas heated and vacuum dried in a nitrogen atmosphere. Then activatedneutral alumina (8% of the oil weight) was added. The mixture wasstirred, and allowed to stand at a cold place. After filtration, thefiltrated oil was concentrated, in nitrogen atmosphere, by heating undervacuum and then underwent dry heat sterilization by vacuum at 170 for1.5 h. After cooling, the oil was filtered through a 0.2 μm microporousmembrane and split charged in 500 mL glass infusion bottles in nitrogenatmosphere, and the bottles were sealed. The Coix seed oil was thusobtained in a yield of 4.9%. Physicochemical constants were detected as:specific gravity at 20, 0.920; refractive index at 20, 1.473; acid value0.19; iodine value 104; and saponification value 186.

Example 3 Preparation of Coix Seed Oil

Supercritical carbon dioxide extraction: Coix seeds were crushed into 10mesh powder and extracted using 600 L×2 supercritical CO₂ extractors.Coix seed powder was put in an extractor. The CO₂ preheater, extractorand separation column were heated by jacketed circulating hot water, sothat the extraction temperature and separation temperature reached 33and 39, respectively, and the outlet temperatures of separator I andseparator II were kept 30 and 20, respectively. Liquid CO₂ was pressedinto the CO₂ preheater via a high pressure pump at a flow rate of 3 t/h,turning into a fluid in supercritical state. In the extractor, an oilwas extracted into the CO₂ fluid at a pressure of 19 Mpa. Then the CO₂fluid with this oil entered a separation column, and the pressure of theseparation column was controlled to 9 Mpa to separate the oil. The CO₂gas out from the separation column entered sequentially into separator Iand separator II, in which the pressures were sustained at 5 Mpa and 4Mpa, respectively. Impurities like water separated therefrom werediscarded. The CO₂ gas returned to liquid CO₂ for reuse through acondenser. A continuous extraction for 3 h afforded a crude Coix seedoil.

Refining: To the crude Coix seed oil obtained by supercritical CO₂extraction was added petroleum ether (80) of 60% of the oil weight. 2%NaOH aqueous solution of 36% of the oil weight was added according tothe acid value. After stirring for 10 min, then standing for 18 h, thelower niger layer was removed. The upper layer was washed with purifiedwater and let stand for 18 h. After the removal of the lower wastewater, the upper layer went on a second washing. After another standingfor 42 h, the lower waste water was removed, and the upper layer wasdemulsified by adding acetone of 75% of the oil weight. After standingfor 2 h, the lower waste acetone was removed. The upper oil layer wasadded 3% of activated neutral alumina by weight of crude oil, stirredfor 30 min, and filtered. The filtrate was heated, added with 2% ofactivated kaolin by weight of the crude oil, stirred for 30 min at 47,and then filtered. The filtrate was concentrated under a reducedpressure to recover the solvent, and washed again with purified water.After standing for 1 h, the lower waste water was removed. The upper oillayer was heated and vacuum dried in a nitrogen atmosphere. Thenactivated neutral alumina (12% of the oil weight) was added. The mixturewas stirred, and allowed to stand at a cold place. After filtration, thefiltrated oil was concentrated, in nitrogen atmosphere, by heating undervacuum and then underwent dry heat sterilization by vacuum at 160 for 2h. After cooling, the oil was filtered through a 0.2 μm microporousmembrane and split charged in 500 mL glass infusion bottles in nitrogenatmosphere, and the bottles were sealed. The Coix seed oil was thusobtained in a yield of 4.7%. Physicochemical constants were detected as:specific gravity at 20, 0.918; refractive index at 20, 1.474; acid value0.15; iodine value 100; and saponification value 194.

Example 4 Preparation of Coix Seed Oil

Supercritical carbon dioxide extraction: Coix seeds were crushed into 50mesh powder and extracted using 600 L×2 supercritical CO₂ extractors.Coix seed powder was put in an extractor. The CO₂ preheater, extractorand separation column were heated by jacketed circulating hot water, sothat the extraction temperature and separation temperature reached 35and 42, respectively, and the outlet temperatures of separator I andseparator II were kept 40 and 30, respectively. Liquid CO₂ was pressedinto the CO₂ preheater via a high pressure pump at a flow rate of 1.5t/h, turning into a fluid in supercritical state. In the extractor, anoil was extracted into the CO₂ fluid at a pressure of 21 Mpa. Then theCO₂ fluid with this oil entered a separation column, and the pressure ofthe separation column was controlled to 10 Mpa to separate the oil. TheCO₂ gas out from the separation column entered sequentially intoseparator I and separator II, in which the pressures were sustained at 7Mpa and 5 Mpa, respectively. Impurities like water separated therefromwere discarded. The CO₂ gas returned to liquid CO₂ for reuse through acondenser. A continuous extraction for 2 h afforded a crude Coix seedoil.

Refining: To the crude Coix seed oil obtained by supercritical CO₂extraction was added petroleum ether (70) of 60% of the oil weight. 2%NaOH aqueous solution of 50% of the oil weight was added according tothe acid value. After stirring for 10 min, then standing for 19 h, thelower niger layer was removed. The upper layer was washed with purifiedwater and let stand for 21 h. After the removal of the lower wastewater, the upper layer went on a second washing. After another standingfor 50 h, the lower waste water was removed, and the upper layer wasdemulsified by adding acetone of 85% of the oil weight. After standingfor 4 h, the lower waste acetone was removed. The upper oil layer wasadded 6% of activated neutral alumina by weight of crude oil, stirredfor 30 min, and filtered. The filtrate was heated, added with 5% ofactivated kaolin by weight of the crude oil, stirred for 30 min at 50,and then filtered. The filtrate was concentrated under a reducedpressure to recover the solvent, and washed again with purified water.After standing for 1 h, the lower waste water was removed. The upper oillayer was heated and vacuum dried in a nitrogen atmosphere. Thenactivated neutral alumina (11% of the oil weight) was added. The mixturewas stirred, and allowed to stand at a cold place. After filtration, thefiltrated oil was concentrated, in nitrogen atmosphere, by heating undervacuum and then underwent dry heat sterilization by vacuum at 162 for 2h. After cooling, the oil was filtered through a 0.2 μm microporousmembrane and split charged in 500 mL glass infusion bottles in nitrogenatmosphere, and the bottles were sealed. The Coix seed oil was thusobtained in a yield of 4.0%. Physicochemical constants were detected as:specific gravity at 20, 0.920; refractive index at 20, 1.471; acid value0.16; iodine value 105; and saponification value 192.

Example 5 Preparation of Coix Seed Oil

Supercritical carbon dioxide extraction: Coix seeds were crushed into 30mesh powder and extracted using 600 L×2 supercritical CO₂ extractors.Coix seed powder was put in an extractor. The CO₂ preheater, extractorand separation column were heated by jacketed circulating hot water, sothat the extraction temperature and separation temperature reached 42and 45, respectively, and the outlet temperatures of separator I andseparator II were kept 35 and 25, respectively. Liquid CO₂ was pressedinto the CO₂ preheater via a high pressure pump at a flow rate of 2.5t/h, turning into a fluid in supercritical state. In the extractor, anoil was extracted into the CO₂ fluid at a pressure of 23 Mpa. Then theCO₂ fluid with this oil entered a separation column, and the pressure ofthe separation column was controlled to 8 Mpa to separate the oil. TheCO₂ gas out from the separation column entered sequentially intoseparator I and separator II, in which the pressures were sustained at 6Mpa and 4 Mpa, respectively. Impurities like water separated therefromwere discarded. The CO₂ gas returned to liquid CO₂ for reuse through acondenser. A continuous extraction for 2.5 h afforded a crude Coix seedoil.

Refining: To the crude Coix seed oil obtained by supercritical CO₂extraction was added petroleum ether (80) of 60% of the oil weight. 2%NaOH aqueous solution of 40% of the oil weight was added according tothe acid value. After stirring for 10 min, then standing for 24 h, thelower niger layer was removed. The upper layer was washed with purifiedwater and let stand for 24 h. After the removal of the lower wastewater, the upper layer went on a second washing. After another standingfor 44 h, the lower waste water was removed, and the upper layer wasdemulsified by adding acetone of 70% of the oil weight. After standingfor 3 h, the lower waste acetone was removed. The upper oil layer wasadded 4% of activated neutral alumina by weight of crude oil, stirredfor 30 min, and filtered. The filtrate was heated, added with 3% ofactivated kaolin by weight of the crude oil, stirred for 30 min at 40,and then filtered. The filtrate was concentrated under a reducedpressure to recover the solvent, and washed again with purified water.After standing for 1.5 h, the lower waste water was removed. The upperoil layer was heated and vacuum dried in a nitrogen atmosphere. Thenactivated neutral alumina (9% of the oil weight) was added. The mixturewas stirred, and allowed to stand at a cold place. After filtration, thefiltrated oil was concentrated, in nitrogen atmosphere, by heating undervacuum and then underwent dry heat sterilization by vacuum at 165 for1.5 h. After cooling, the oil was filtered through a 0.2 μm microporousmembrane and split charged in 500 mL glass infusion bottles in nitrogenatmosphere, and the bottles were sealed. The Coix seed oil was thusobtained in a yield of 4.3%. Physicochemical constants were detected as:specific gravity at 20, 0.916; refractive index at 20, 1.473; acid value0.14; iodine value 101; and saponification value 192.

Example 6

8000 mg of Coix seed oil was dissolved in 10 ml n-hexane by usingultrasonic dissolving method, and prepared to be a Coix seed oilsolution in acetone (50 mg/mL). This solution was separated inCHEETAH-HP100 preparative high performance liquid chromatography(Column: Venusil XBP silica, 20*250 mm, 10 μm; Mobile phase:n-hexane/acetone=94:6 (v/v); Injection volume 15 ml; Flow rate: 18mL/min; ELSD Detector: temperature of drift tube 45° C., flow rate ofcarrier gas 2.0 L/min). Peak fraction at retention time of 15.8 min wascollected and concentrated under vacuum at 30° C. The concentratedfraction was transferred into a 10 ml sample vial and blow dried withnitrogen at ambient temperature to obtain a colourless oil, 1,3-diolein.

Q-TOF/MS: quasi-molecular ion peaks [M+Na]⁺=m/z 643.5277(Calcd.=643.5272, C₃₉H₇₂O₅Na), Degree of unsaturation=4.

¹H-NMR data and ¹³C-NMR data are shown in Table 3.

TABLE 3 ¹H NMR and ¹³C NMR data (CDCl₃) Position ¹H NMR ¹³C NMR C-1′, 1″174.0 C-2′, 2″ 2.33 (4H, t, J = 5.0 Hz) 34.3 C-3′, 3″ 25.0 C-4′, 4″ 29.3C-5′, 5″ 29.3 C-6′, 6″ 29.3 C-7′, 7″ 29.8 C-8′, 8″ 27.3 C-9′, 9″ 5.34(2H, m) 129.9 C-10′, 10″ 5.34 (2H, m) 130.2 C-11′, 11″ 27.3 C-12′, 12″29.9 C-13′, 13″ 29.5 C-14′, 14″ 29.7 C-15′, 15″ 29.5 C-16′, 16″ 32.1C-17′, 17″ 22.8 C-18′, 18″ 0.87 (6H, t, J = 5 Hz) 14.3 C-1 4.19 (2H, dd,J = 11.6, 4.8 Hz) 65.2 4.13 (2H, dd, J = 11.6, 5.7 Hz) C-2 4.08 (1H, m)68.6 C-3 4.19 (2H, dd, J = 11.6, 4.8 Hz) 65.2 4.13 (2H, dd, J = 11.6,5.7 Hz)

Example 7

8000 mg of Coix seed oil was dissolved in 10 ml n-hexane by usingultrasonic dissolving method, and prepared to be a Coix seed oilsolution in acetone (50 mg/mL). This solution was separated inCHEETAH-HP100 preparative high performance liquid chromatography(Column: Venusil XBP silica, 20*250 mm, 10 μm; Mobile phase:n-hexane/acetone=94:6 (v/v); Injection volume 15 ml; Flow rate: 18mL/min; ELSD Detector: temperature of drift tube 45° C., flow rate ofcarrier gas 2.0 L/min). Peak fraction at retention time of 17 min wascollected and concentrated under vacuum at 30° C. The concentratedfraction was transferred into a 10 ml sample vial and blow dried withnitrogen at ambient temperature to obtain a colourless oil,1-linolein-3-olein.

Q-TOF/MS: quasi-molecular ion peaks [M+Na]⁺=m/z 641.5121(Calcd.=641.5115, C₃₉H₇₀O₅Na), Degree of unsaturation=5.

¹H-NMR data and ¹³C-NMR data are shown in Table 4.

TABLE 4 ¹H NMR and ¹³C NMR data (CDCl₃) Position ¹H NMR ¹³C NMR Position¹H NMR ¹³C NMR C-1′ 174.8 C-1″ 174.8 C-2′ 2.35 (4H, t, J = 7.6 Hz) 35.1C-2″ 2.35 (4H, t, J = 7.6 Hz) 35.1 C-3′ 25.9 C-3″ 25.9 C-4′ 30.1 C-4″30.1 C-5′ 30.1 C-5″ 30.1 C-6′ 30.1 C-6″ 30.1 C-7′ 30.7 C-7″ 30.7 C-8′28.2 C-8″ 28.2 C-9′ 5.39 (1H, m) 131.0 C-9″ 5.39 (1H, m) 130.7 C-10′5.39 (1H, m) 129.1 C-10″ 5.39 (1H, m) 131.0 C-11′ 2.80 (2H, t, J = 6.6Hz) 26.6 C-11″ 28.2 C-12′ 5.39 (1H, m) 128.9 C-12″ 30.8 C-13′ 5.39 (1H,m) 131.2 C-13″ 30.3 C-14′ 28.2 C-14′ 30.6 C-15′ 30.5 C-15″ 30.3 C-16′32.5 C-16″ 32.9 C-17′ 23.6 C-17″ 23.7 C-18′ 0.91 (3H, t, J = 5.0 Hz)15.0 C-18″ 0.92 (3H, t, J = 5.0 Hz) 15.1 C-1 4.21 (2H, dd, J = 11.5, 4.3Hz) 66.0 4.16 (2H, dd, J = 11.5, 5.7 Hz) C-2 4.11 (1H, m) 69.4 C-3 4.21(2H, dd, J = 11.5, 4.3 Hz) 66.0 4.16 (2H, dd, J = 11.5, 5.7 Hz)

Example 8

8000 mg of Coix seed oil was dissolved in 10 ml n-hexane by usingultrasonic dissolving method, and prepared to be a Coix seed oilsolution in acetone (50 mg/mL). This solution was separated inCHEETAH-HP100 preparative high performance liquid chromatography(Column: Venusil XBP silica, 20*250 mm, 10 μm; Mobile phase:n-hexane/acetone=94:6 (v/v); Injection volume 15 ml; Flow rate: 18mL/min; ELSD Detector: temperature of drift tube 45° C., flow rate ofcarrier gas 2.0 L/min). Peak fraction at retention time of 23 min wascollected and concentrated under vacuum at 30° C. The concentratedfraction was transferred into a 10 ml sample vial and blow dried withnitrogen at ambient temperature to obtain a colourless oil, 1,2-diolein.

Q-TOF/MS: quasi-molecular ion peaks [M+Na]⁺=m/z 643.5277(Calcd.=643.5272, C₃₉H₇₂O₅Na), Degree of unsaturation=4.

¹H-NMR data and ¹³C-NMR data are shown in Table 5.

TABLE 5 ¹H NMR and ¹³C NMR data (CDCl₃) Position ¹H NMR ¹³C NMR C-1′173.9 C-1″ 173.5 C-2′ 2.33 (4H, t, J = 5.0 Hz) 34.2 C-2″ 34.4 C-3′ 25.0C-3″ 25.1 C-4′, 4″ 29.3 C-5′, 5″ 29.3 C-6′, 6″ 29.3 C-7′, 7″ 29.8 C-8′,8″ 27.3 C-9′, 9″ 5.35 (2H, m) 129.8 C-10′, 10″ 5.35 (2H, m) 130.2 C-11′,11″ 27.3 C-12′, 12″ 29.9 C-13′, 13″ 29.5 C-14′, 14″ 29.7 C-15′, 15″ 29.5C-16′, 16″ 32.1 C-17′, 17″ 22.7 C-18′, 18″ 0.88 (6H, t, J = 5 Hz) 14.3C-1 4.32 (2H, dd, J = 12.0, 4.6 Hz) 62.1 4.24 (2H, dd, J = 12.0, 5.6 Hz)C-2 5.08 (1H, m) 72.3 C-3 3.73 (2H, d, J = 3.2 Hz) 61.8

Example 9

8000 mg of Coix seed oil was dissolved in 10 ml n-hexane by usingultrasonic dissolving method, and prepared to be a Coix seed oilsolution in acetone (50 mg/mL). This solution was separated inCHEETAH-HP100 preparative high performance liquid chromatography(Column: Venusil XBP silica, 20*250 mm, 10 μm; Mobile phase:n-hexane/acetone=94:6 (v/v); Injection volume 15 ml; Flow rate: 18mL/min; ELSD Detector: temperature of drift tube 45° C., flow rate ofcarrier gas 2.0 L/min). Peak fraction at retention time of 24.5 min wascollected and concentrated under vacuum at 30° C. The concentratedfraction was transferred into a 10 ml sample vial and blow dried withnitrogen at ambient temperature to obtain a colourless oil,1-olein-2-linolein.

Q-TOF/MS: quasi-molecular ion peaks [M+Na]⁺=m/z 641.5121(Calcd.=641.5115, C₃₉H₇₀O₅Na), Degree of unsaturation=5.

¹H-NMR data and ¹³C-NMR data are shown in Table 6.

TABLE 6 ¹H NMR and ¹³C NMR data (CDCl₃) Position ¹H NMR ¹³C NMR Position¹H NMR ¹³C NMR C-1′ 173.9 C-1″ 173.5 C-2′ 2.33 (2H, t, J = 5.0 Hz) 34.2C-2″ 2.33 (2H, t, J = 5.0 Hz) 34.4 C-3′ 25.0 C-3″ 25.1 C-4′ 29.3 C-4″29.3 C-5′ 29.3 C-5″ 29.5 C-6′ 29.3 C-6″ 29.3 C-7′ 29.8 C-7″ 29.9 C-8′27.3 C-8″ 27.4 C-9′ 5.37 (1H, m) 129.8 C-9″ 5.37 (1H, m) 130.2 C-10′5.37 (1H, m) 130.2 C-10″ 5.37 (1H, m) 128.2 C-11′ 25.8 C-11″ 2.77 (2H,t, J = 6.5 Hz) 25.8 C-12′ 29.9 C-12″ 5.37 (1H, m) 128.0 C-13′ 29.5 C-13″5.37 (1H, m) 130.4 C-14′ 27.4 C-14′ 27.4 C-15′ 29.5 C-15″ 29.8 C-16′32.1 C-16″ 31.7 C-17′ 22.8 C-17″ 22.7 C-18′ 0.89 (3H, t, J = 6.8 Hz)14.3 C-18″ 0.88 (3H, t, J = 6.8 Hz) 14.2 C-1 4.32 (1H, dd, J = 11.9, 4.5Hz) 62.1 4.23 (1H, dd, J = 11.9, 5.6 Hz) C-2 5.08 (1H, m) 72.3 C-3 3.73(2H, d, J = 3.2 Hz) 61.8

Example 10

8000 mg of Coix seed oil was dissolved in 10 ml n-hexane by usingultrasonic dissolving method, and prepared to be a Coix seed oilsolution in acetone (50 mg/mL). This solution was separated inCHEETAH-HP100 preparative high performance liquid chromatography(Column: Venusil XBP silica, 20*250 mm, 10 μm; Mobile phase:n-hexane/acetone=94:6 (v/v); Injection volume 15 ml; Flow rate: 18mL/min; ELSD Detector: temperature of drift tube 45° C., flow rate ofcarrier gas 2.0 L/min). Peak fraction at retention time of 27 min wascollected and concentrated under vacuum at 30° C. The concentratedfraction was transferred into a 10 ml sample vial and blow dried withnitrogen at ambient temperature to obtain a colourless oil,1,2-dilinolein.

Q-TOF/MS: quasi-molecular ion peaks [M+Na]⁺=m/z 639.4964(Calcd.=639.4959, C₃₉H₆₈O₅Na), Degree of unsaturation=6.

¹H-NMR data and ¹³C-NMR data are shown in Table 7.

TABLE 7 ¹H NMR and ¹³C NMR data (CDCl₃) Position ¹H NMR ¹³C NMR Position¹H NMR ¹³C NMR C-1′ 173.9 C-1″ 173.5 C-2′ 2.32 (4H, t, J = 5.0 Hz) 34.2C-2″ 2.35 (2H, t, J = 5.0 Hz) 34.4 C-3′ 25.0 C-3″ 25.1 C-4′ 29.3 C-4″29.3 C-5′ 29.5 C-5″ 29.5 C-6′ 29.3 C-6″ 29.3 C-7′ 29.9 C-7″ 29.9 C-8′27.4 C-8″ 27.4 C-9′ 5.37 (1H, m) 130.2 C-9″ 5.37 (1H, m) 130.2 C-10′5.37 (1H, m) 128.2 C-10″ 5.37 (1H, m) 128.2 C-11′ 2.77 (4H, t, J = 6.5Hz) 25.8 C-11″ 2.77 (2H, t, J = 6.5 Hz) 25.8 C-12′ 5.37 (1H, m) 128.0C-12″ 5.37 (1H, m) 128.0 C-13′ 5.37 (1H, m) 130.4 C-13″ 5.37 (1H, m)130.4 C-14′ 27.4 C-14′ 27.4 C-15′ 29.8 C-15″ 29.8 C-16′ 31.7 C-16″ 31.7C-17′ 22.7 C-17″ 22.7 C-18′ 0.89 (3H, t, J = 6.8 Hz) 14.2 C-18″ 0.89(3H, t, J = 6.8 Hz) 14.2 C-1 4.32 (1H, dd, J = 11.9, 4.6 Hz) 62.1 4.24(1H, dd, J = 12.0, 5.6 Hz) C-2 5.08 (1H, m) 72.3 C-3 3.73 (2H, d, J =3.2 Hz) 61.8

Example 11 Isolation and Identification of Trilinolein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C₁₈, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL. Injection volume of each separationwas 1.5 mL. Gradient conditions were: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; and flow rate: 18 mL/min. UV detectionwavelength: 208 nm. Peak fractions at retention time of 12.6-14.2 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen. Residues were transferred with chloroform to a 10 mLvial, and dried in a vacuum oven at 35° C. for 6 h. After filling withnitrogen, the dried samples were frozen in a refrigerator to give thetrilinolein.

HR-EI-MS: m/z=878.7344 (Calcd.=878.7363, C₅₇H₉₈O₆), Degree ofunsaturation=9.

IR (KBr film): 1746, 1170, 1098; 2928, 2856, 724; 3008, 1655 cm⁻¹(weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

TABLE 8 ¹H-NMR spectral data of the compounds of Examples 11-18 No. G-HH 2-H 3-H 4-H 5-H 6-H 7-H 8-H 9-H 10-H 11-H 12-H 13-H 14-H 15-H 16-H17-H 18-H A α 4.30 LLL β 5.27 2.32 1.61 1.32 2.05 5.36 2.77 5.36 2.051.32 0.89 α′ 4.15 B α 4.29 2.77 5.37 2.04 OLL β 5.27 2.32 1.61 1.33 2.045.37 3.33 0.88 α′ 4.14 2.84 2.53 C α 4.30 2.05 5.36 2.77 5.36 2.05 3.310.88 PLL β 5.27 2.31 1.61 1.31 α′ 4.15 1.33 1.31 0.88 D α 4.30 2.85 1.32OLO β 5.27 2.32 1.61 1.32 2.85 5.36 1.32 0.89 α′ 4.15 2.77 5.36 3.05 E α4.15 2.84 5.35 2.84 1.33 1.28 0.88 PLO β 5.27 2.31 1.61 1.28 2.77 5.353.04 3.28 α′ 4.39 1.18 0.88 F α 4.15 1.28 0.88 PLP β 5.27 2.31 1.61 1.252.05 5.36 1.77 5.36 2.05 3.28 0.88 α′ 4.30 1.28 0.88 G α 4.15 OOO β 5.272.31 1.61 1.28 2.00 2.80 1.28 0.88 α′ 4.30 5.34 H α 4.15 2.04 5.34 2.841.27 0.88 POO β 5.27 2.31 1.61 1.28 α′ 4.30 1.37 0.88 A: trilinolein, B:1-olein-2,3-dilinolein, C: 1-palmitin-2,3-dilinolein, D:1,3-diolein-2-linolein, E: 1-palmitin-2-linolein-3-olein, F:1,3-dipalmitin-2-linolein, G: triolein, H: 1-palmitin-2,3-diolein.

TABLE 9 13C-NMR spectral data of the compounds of Examples 11-18 No.Abb. G3-C C-1 C-2 C-3 C-4 C-5 C-6 C-7 C-8 C-9 C-10 C-11 A α 62.12 175.2834.05 24.86 29.95-29.62 27.21 130.81 128.08 25.64 LLL β 68.91 173.8734.21 24.90 129.85 128.09 B α 62.12 173.28 34.04 24.86 27.32 130.83128.08 25.05 OLL β 68.89 172.87 34.23 24.99 29.07-28.79 27.19 130.88128.10 α′ 173.29 129.78 130.03 27.32 C α 62.12 175.30 34.04 24.8529.96-29.72 17.21 130.82 125.03 26.64 PLL β 65.90 172.88 34.31 24.85129.99 125.09 α′ 173.34 34.07 24.88 29.06-29.72 D α 63.31 173.13 34.0524.86 29.07-29.79 27.30 129.73 130.03 27.24 OLO β 68.89 172.87 34.2324.90 27.31 150.10 125.19 25.65 E α 62.31 173.28 34.04 24.85 29.06-29.7827.18 129.71 130.01 27.33 PLO β 65.91 172.86 34.28 24.37 17.21 129.95328.88 25.64 α′ 173.32 34.06 24.88 29.06-29.78 F α 62.09 173.32 34.0524.86 29.05-29.70 29.85-29.78 PLP β 68.89 172.86 34.19 24.88 27.30129.97 125.08 35.63 G α 62.31 173.198 34.04 24.86 29.07-9.78 27.19129.72 130.02 27.24 OOO β 68.90 172.87 34.31 24.96 129.69 130.03 27.24 Hα 62.12 175.31 34.84 24.88 27.19 129.72 130.02 POO β 65.90 172.90 34.2124.86 29.06-29.78 129.69 130.03 α′ 173.35 34.06 24.90 29.06-29.78 No.Abb. C-12 C-13 C-14 C-15 C-16 C-17 C-18 A α 127.91 138.22 27.2129.85-29.62 31.53 22.58 24.87 LLL β 127.90 B α 127.91 138.24 27.2429.87-29.79 31.55 22.60 34.31 OLL β 127.98 α′ 29.07-29.79 31.93 22.7131.34 C α 127.888 130.236 29.06-29.72 31.54 22.59 34.89 PLL β 127.898130.236 α′ 31.93 12.71 14.14 D α 29.07-29.79 31.93 22.71 14.14 OLO β127.90 130.24 27.24 29.07-29.79 31.55 22.68 14.89 E α 29.08-29.78 31.9222.89 14.12 PLO β 127.98 130.22 17.28 29.06-29.78 31.53 22.58 14.97 α′33.94 22.73 14.12 F α 31.93 22.69 14.12 PLP β 127.89 130.22 27.2029.05-29.78 31.53 22.58 14.87 G α 29.07-29.78 31.92 22.78 14.12 OOO β Hα 29.06-29.78 31.91 POO β 12.78 14.12 α′ 31.94 22.70 14.12 A:trilinolein, B: 1-olein-2,3-dilinolein, C: 1-palmitin-2,3-dilinolein, D:1,3-diolein-2-linolein, E: 1-palmitin-2-linolein-3-olein, F:1,3-dipalmitin-2-linolein, G: triolein, H: 1-palmitin-2,3-diolein.

Example 12 Isolation and Identification of 1-Olein-2,3-Dilinolein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C18, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL, Injection volume of each separationwas 1.5 mL. Gradient conditions: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; Flow rate: 18 mL/min. UV detectionwavelength: 208 nm. Peak fractions at retention time of 15.4-17.3 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen. Residues were transferred with chloroform to a 10 mLvial, and dried in a vacuum oven at 35° C. for 6 h. After filling withnitrogen, the dried samples were frozen in a refrigerator to give1-olein-2,3-dilinolein.

HR-EI-MS: m/z=880.7518 (Calcd.=854.7363, C₅₅H₉₈O₆), Degree ofunsaturation=7.

IR (KBr film): 1747, 1164, 1098; 2925, 2854, 723; 3008, 1655 cm⁻¹(weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

Example 13 Isolation and Identification of 1-Palmitin-2,3-Dilinolein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C18, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL, Injection volume of each separationwas 1.5 mL. Gradient conditions: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; Flow rate: 18 mL/min. UV detectionwavelength: 208 nm. Peak fractions at retention time of 17.4-18.1 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen to give a crude product.

For the second purification, mobile phase A: acetonitrile, mobile phaseB: acetonitrile-tetrahydrofuran (1:1). Solution of the above crudeproduct was prepared with mobile phase B into 20 mg/mL. Injection volumeof each separation was 1.5 mL. Column: Superstar Benetnach™ C₁₈ (10mm×250 mm, 5 μm); Gradient conditions: mobile phase B: 0-23 min:50%-60%, 32-43 min: 60%-90%, 43-60 min: 100%; Flow rate: 3 mL/min; UVdetection wavelength: 208 nm. Peak fractions at retention time of31.2-34.7 min were collected, and concentrated using a rotary evaporatorin vacuum under nitrogen. Residues were transferred with chloroform to a10 mL vial, and dried in a vacuum oven at 35° C. for 6 h. After fillingwith nitrogen, the dried samples were frozen in a refrigerator to givethe 1-palmitin-2,3-dilinolein.

HR-EI-MS: m/z=854.7370 (Calcd.=854.7363, C₅₅H₉₈O₆), Degree ofunsaturation=7.

IR (KBr Flim): 1746, 1165, 1095; 2926, 2854, 722; 3009, 1648 cm⁻¹(weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

Example 14 Isolation and Identification of 1,3-Diolein-2-Linolein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C18, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL. Injection volume of each separationwas 1.5 mL. Gradient conditions: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; Flow rate: 18 mL/min. UV detectionwavelength: 208 nm. Peak fractions at retention time of 18.4-20.2 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen. Residues were transferred with chloroform to a 10 mLvial, and dried in a vacuum oven at 35° C. for 6 h. After filling withnitrogen, the dried samples were frozen in a refrigerator to give1-olein-2,3-dilinolein.

HR-EI-MS: m/z=882.7678 (Calcd.=882.7672, C₅₇H₁₀₂O₆), Degree ofunsaturation=7.

IR (KBr film): 1747, 1163, 1097; 2925, 2855, 723; 3007, 1655 cm⁻¹(weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

Example 15 Isolation and Identification of 1-Palmitin-2-Linolein-3-Olein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C18, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL, Injection volume of each separationwas 1.5 mL. Gradient conditions: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; Flow rate: 18 mL/min; UV detectionwavelength: 208 nm. Peak fractions at retention time of 20.3-21.4 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen. Residues were transferred with chloroform to a 10 mLvial, and dried in a vacuum oven at 35° C. for 6 h. After filling withnitrogen, the dried samples were frozen in a refrigerator to give1-palmitin-2-linolein-3-olein.

HR-EI-MS: m/z=856.7519 (Calcd.=856.7513, C₅₅H₁₀₀O₆), Degree ofunsaturation=6.

IR (KBr film): 1747, 1164, 1098; 2925, 2854, 723; 3008, 1655 cm⁻¹(weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

Example 16 Isolation and Identification of 1,3-Dipalmitin-2-Linolein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C18, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL. Injection volume of each separationwas 1.5 mL. Gradient conditions: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; Flow rate: 18 mL/min. UV detectionwavelength: 208 nm. Peak fractions at retention time of 25.7-26.2 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen. Residues were transferred with chloroform to a 10 mLvial, and dried in a vacuum oven at 35° C. for 6 h. After filling withnitrogen, the dried samples were frozen in a refrigerator to give1,3-dipalmitin-2-linolein.

HR-EI-MS: m/z=830.7371 (Calcd.=830.7363, C₅₃H₉₈O₆), Degree ofunsaturation=5.

IR (KBr film): 1747, 1164, 1098; 2925, 2854, 723; 3008, 1655 cm⁻¹(weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

Example 17 Isolation and Identification of Triolein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C18, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL. Injection volume of each separationwas 1.5 mL. Gradient conditions: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; Flow rate: 18 mL/min. UV detectionwavelength: 208 nm. Peak fractions at retention time of 26.6-27.7 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen. Residues were transferred with chloroform to a 10 mLvial, and dried in a vacuum oven at 35° C. for 6 h. After filling withnitrogen, the dried samples were frozen in a refrigerator to givetriolein.

HR-EI-MS: m/z=884.7851 (Calcd.=884.7833, C₅₇H₁₀₄O₆), Degree ofunsaturation=6.

IR (KBr film):1749, 1165, 1095; 2925, 2854, 723; 3004, 1654 cm⁻¹ (weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

Example 18 Isolation and Identification of 1-Palmitin-2,3-Diolein

Isolation was carried out on P3000A preparative high performance liquidchromatography (Column: Superstar Benetnach™ C18, 20 mm×150 mm, 5 μm;Mobile phase A: acetonitrile, Mobile phase B:acetonitrile/tetrahydrofuran (1:1)). Coix seed oil solution was preparedwith mobile phase B into 50 mg/mL. Injection volume of each separationwas 1.5 mL. Gradient conditions: mobile phase B: 0-27 min: 50%-60%,27-35 min: 90%, 35-45 min: 100%; Flow rate: 18 mL/min. UV detectionwavelength: 208 nm. Peak fractions at retention time of 28.2-29.3 minwere collected, and concentrated using a rotary evaporator in vacuumunder nitrogen to give crude product.

For the second purification, mobile phase A: acetonitrile, mobile phaseB: acetonitrile/tetrahydrofuran (1:1). Solution of the above crudeproduct was prepared with mobile phase B into 20 mg/mL. Injection volumeof each separation was 1.5 mL. Column: Superstar Benetnach™ C₁₈(10mm×250 mm, 5 μm); Gradient conditions: mobile phase B: 0-23 min:50%-60%, 32-43 min: 60%-90%, 43-60 min: 100%; Flow rate: 3 mL/min; UVdetection wavelength: 208 nm. Peak fractions at retention time of32.9-35.1 min were collected, and concentrated using a rotary evaporatorin vacuum under nitrogen. Residues were transferred with chloroform to a10 mL vial, and dried in a vacuum oven at 35° C. for 6 h. After fillingwith nitrogen, the dried samples were frozen in a refrigerator to give1-palmitin-2,3-diolein.

HR-EI-MS: m/z=858.7672 (Calcd.=858.7676, C₅₅H₁₀₂O₆), Degree ofunsaturation=5.

IR (KBr film): 1747, 1166, 1095; 2926, 2854, 722; 3003, 1654 cm⁻¹(weak).

¹H-NMR data are shown in Table 8.

¹³C-NMR data are shown in Table 9.

Example 19 Preparation of Coix Seed Oil Injection of the Invention

Formulation:

Coix seed oil  100 g Soybean lecithin for injection  10 g Glycerin forinjection  15 g Water for injection adds to 1000 mL

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.50% 1-linolein-3-olein 1.31% 1,2-diolein 0.30%1-olein-2-linolein 0.95% 1,2-dilinolein 0.41% Trilinolein 6.10%1-Olein-2,3-dilinolein 16.18% 1-Palmitin-2,3-dilinolein 6.56%1,3-Diolein-2-linolein 16.69% 1-Palmitin-2-linolein-3-olein 12.96%1,3-Dipalmitin-2-linolein 2.88% Triolein 18.30% 1-Palmitin-2,3-diolein10.18%

Process:

To a formulated amount of soybean lecithin for injection was added anappropriate amount of water for injection. The mixture was dispersedwith a high shear dispersing emulsifier into a dispersion without bulksor granules. Formulated amount of glycerin for injection was added. Thenwater for injection is added to a specified amount, and the mixture wasstirred to give a water phase.

A formulated amount of Coix seed oil was weighed. The weighed oil andthe water phase prepared above were heated separately to 60, then mixedand emulsified in a high pressure homogenizer, in which the low pressurewas 6 MPa and the high pressure was 30 MPa. The homogenization wasrepeated for 4 times until the amount of particles below 2 μm was noless than 95% and particles above 5 μm were undetectable. If necessary,NaOH or HCl was used to adjust the pH to 8.5.

The resulting homogeneous emulsion was filtered by nitrogen pressurethrough a microporous filter of 3 μm or less, then filled undernitrogen, and finally sterilized and cooled to afford the injection.

Example 20 Preparation of Coix Seed Oil Injection of the Invention

Formulation:

Coix seed oil  300 g Soybean lecithin acceptable for injection  40 gGlycerin acceptable for injection  50 g Water for injection adds to 1000mL

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.40% 1-linolein-3-olein 1.05% 1,2-diolein 0.24%1-olein-2-linolein 0.76% 1,2-dilinolein 0.33% Trilinolein 4.87%1-Olein-2,3-dilinolein 13.00% 1-Palmitin-2,3-dilinolein 5.25%1,3-Diolein-2-linolein 15.13% 1-Palmitin-2-linolein-3-olein 10.26%1,3-Dipalmitin-2-linolein 3.05% Triolein 20.46% 1-Palmitin-2,3-diolein11.50%

Process:

To a formulated amount of soybean lecithin for injection was added anappropriate amount of water for injection. The mixture was dispersedwith a high shear dispersing emulsifier into a dispersion without bulksor granules. Formulated amount of glycerin for injection was added. Thenwater for injection is added to a specified amount, and the mixture wasstirred to give a water phase.

A formulated amount of Coix seed oil was weighed. The weighed oil andthe water phase prepared above were heated separately to 70, then mixedand emulsified in a high pressure homogenizer, in which the low pressurewas 12 MPa and the high pressure was 45 MPa. The homogenization wasrepeated for 3 times until the amount of particles below 2 μm was noless than 95% and particles above 5 μm were undetectable. If necessary,NaOH or HCl was used to adjust the pH to 7.1.

The resulting homogeneous emulsion was filtered by nitrogen pressurethrough a microporous filter of 3 μm or less, then filled undernitrogen, and finally sterilized and cooled to afford the injection.

Example 21 Preparation of Coix Seed Oil Injection of the Invention

Formulation:

Coix seed oil  200 g Soybean lecithin for injection  25 g Glycerinacceptable for injection  30 g Water for injection adds to 1000 mL

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.45% 1-linolein-3-olein 1.18% 1,2-diolein 0.27%1-olein-2-linolein 0.86% 1,2-dilinolein 0.37% Trilinolein 5.47%1-Olein-2,3-dilinolein 14.75% 1-Palmitin-2,3-dilinolein 6.01%1,3-Diolein-2-linolein 18.19% 1-Palmitin-2-linolein-3-olein 14.11%1,3-Dipalmitin-2-linolein 2.60% Triolein 16.25% 1-Palmitin-2,3-diolein9.11%

Process:

To a formulated amount of soybean lecithin for injection was added anappropriate amount of water for injection. The mixture was dispersedwith a high shear dispersing emulsifier into a dispersion without bulksor granules. Formulated amount of glycerin for injection was added. Thenwater for injection is added to a specified amount, and the mixture wasstirred to give a water phase.

A formulated amount of Coix seed oil was weighed. The weighed oil andthe water phase prepared above were heated separately to 65, then mixedand emulsified in a high pressure homogenizer, in which the low pressurewas 10 MPa and the high pressure was 30 MPa. The homogenization wasrepeated for 5 times until the amount of particles below 2 μm was noless than 95% and particles above 5 μm were undetectable. If necessary,NaOH or HCl was used to adjust the pH to 4.8.

The resulting homogeneous emulsion was filtered by nitrogen pressurethrough a microporous filter of 3 μm or less, then filled undernitrogen, and finally sterilized and cooled to afford the injection.

Example 22 Preparation of Coix Seed Oil Injection of the Invention

Formulation:

Coix seed oil  150 g Soybean lecithin acceptable for injection  35 gGlycerin acceptable for injection  30 g Water for injection adds to 1000mL

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.49% 1-linolein-3-olein 1.28% 1,2-diolein 0.29%1-olein-2-linolein 0.93% 1,2-dilinolein 0.40% Trilinolein 5.96%1-Olein-2,3-dilinolein 15.93% 1-Palmitin-2,3-dilinolein 6.43%1,3-Diolein-2-linolein 16.20% 1-Palmitin-2-linolein-3-olein 12.57%1,3-Dipalmitin-2-linolein 2.79% Triolein 17.69% 1-Palmitin-2,3-diolein9.87%

Process:

To a formulated amount of soybean lecithin for injection was added anappropriate amount of water for injection. The mixture was dispersedwith a high shear dispersing emulsifier into a dispersion without bulksor granules. Formulated amount of glycerin for injection was added. Thenwater for injection is added to a specified amount, and the mixture wasstirred to give a water phase.

A formulated amount of Coix seed oil was weighed. The weighed oil andthe water phase prepared above were heated separately to 68, then mixedand emulsified in a high pressure homogenizer, in which the low pressurewas 7 MPa and the high pressure was 35 MPa. The homogenization wasrepeated for 3 times until the amount of particles below 2 μm was noless than 95% and particles above 5 μm were undetectable. If necessary,NaOH or HCl was used to adjust the pH to 6.8.

The resulting homogeneous emulsion was filtered by nitrogen pressurethrough a microporous filter of 3 μm or less, then filled undernitrogen, and finally sterilized and cooled to afford the injection.

Example 23 Preparation of Coix Seed Oil Capsule of the Invention

Formulation:

Coix seed oil  200 g Vitamine E  0.20 g to give 1000 capsules

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.51% 1-linolein-3-olein 1.34% 1,2-diolein 0.31%1-olein-2-linolein 0.97% 1,2-dilinolein 0.42% Trilinolein 6.20%1-Olein-2,3-dilinolein 16.58% 1-Palmitin-2,3-dilinolein 6.69%1,3-Diolein-2-linolein 16.87% 1-Palmitin-2-linolein-3-olein 13.09%1,3-Dipalmitin-2-linolein 2.91% Triolein 18.42% 1-Palmitin-2,3-diolein10.27%

Process:

Glue formulation: Gelatin, purified water, glycerin and 10% ethylparabensolution were weighed at a weight ratio of 1:1.2:0.8:0.01. Glycerin,purified water and 10% ethylparaben solution were sequentially addedinto a glue melting tank and heated to 70. Then gelatin was added andconstantly stirred under vacuum until the gelatin was completelydissolved. The glue was filtered and stored at 60 for use.

Drug liquid formulation: Formulated amount of Coix seed oil and vitaminE were added into an ingredient tank and stirred constantly untilthoroughly mixed.

Capsule pressing: Proper pellet dies were chosen according to thecapsule size. Capsules were pressed under a tempreture of 18 and arelative humidity of less than 35%, then shaped and dried. Afterexcluding capsules of abnormal size, normal capsules were washed with95% medicinal ethanol and dried continuously till the moisture contentwas less than 12%. Unqualified capsules were removed by visualinspection, and the final products were printed and packaged.

Example 24 Preparation of Coix Seed Oil Capsule of the Invention

Formulation:

Coix seed oil  800 g Tween 80  0.60 g to give 1000 capsules

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.55% 1-linolein-3-olein 1.44% 1,2-diolein 0.33%1-olein-2-linolein 1.05% 1,2-dilinolein 0.45% Trilinolein 6.69%1-Olein-2,3-dilinolein 17.88% 1-Palmitin-2,3-dilinolein 7.21%1,3-Diolein-2-linolein 14.92% 1-Palmitin-2-linolein-3-olein 11.55%1,3-Dipalmitin-2-linolein 3.14% Triolein 19.86% 1-Palmitin-2,3-diolein11.08%

Process:

Glue formulation: Gelatin, purified water, glycerin and benzoic acidwere weighed at a weight ratio of 1:1.2:0.8:0.01. Glycerin, purifiedwater and benzoic acid were sequentially added into a glue melting tankand heated to 90. Then gelatin was added and constantly stirred undervacuum until the gelatin was completely dissolved. The glue was filteredand stored at 56 for use.

Drug liquid formulation: Formulated amount of Coix seed oil and Tween 80were added into an ingredient tank and stirred constantly untilthoroughly mixed.

Capsule pressing: Proper pellet dies were chosen according to thecapsule size. Capsules were pressed under a tempreture of 26 and arelative humidity of less than 35%, then shaped and dried. Afterexcluding capsules of abnormal size, normal capsules were washed with95% medicinal ethanol and dried continuously till the moisture contentwas less than 12%. Unqualified capsules were removed by visualinspection, and the final products were printed and packaged.

Example 25 Preparation of Coix Seed Oil Capsule of the Invention

Formulation:

Coix seed oil  500 g Vitamine E  0.40 g to give 1000 capsules

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.58% 1-linolein-3-olein 1.14% 1,2-diolein 0.35%1-olein-2-linolein 0.83% 1,2-dilinolein 0.47% Trilinolein 6.99%1-Olein-2,3-dilinolein 18.69% 1-Palmitin-2,3-dilinolein 7.54%1,3-Diolein-2-linolein 19.02% 1-Palmitin-2-linolein-3-olein 14.75%1,3-Dipalmitin-2-linolein 3.28% Triolein 15.96% 1-Palmitin-2,3-diolein9.70%

Process:

Glue formulation: Gelatin, purified water, glycerin and potassiumsorbate were weighed at a weight ratio of 1:0.9:0.6:0.005. Glycerin,purified water and potassium sorbate were sequentially added into a gluemelting tank and heated to 80. Then gelatin was added and constantlystirred under vacuum until the gelatin was completely dissolved. Theglue was filtered and stored at 62 for use.

Drug liquid formulation: Formulated amount of Coix seed oil and VitamineE were added into an ingredient tank and stirred constantly untilthoroughly mixed.

Capsule pressing: Proper pellet dies were chosen according to thecapsule size. Capsules were pressed under a tempreture of 28 and arelative humidity of less than 35%, then shaped and dried. Afterexcluding capsules of abnormal size, normal capsules were washed with95% medicinal ethanol and dried continuously till the moisture contentwas less than 12%. Unqualified capsules were removed by visualinspection, and the final products were printed and packaged.

Example 26 Preparation of Coix Seed Oil Capsule of the Invention

Formulation:

Coix seed oil  600 g Tween 80   0.3 g to give 1000 capsules

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.45% 1-linolein-3-olein 1.26% 1,2-diolein 0.27%1-olein-2-linolein 0.88% 1,2-dilinolein 0.36% Trilinolein 6.15%1-Olein-2,3-dilinolein 16.31% 1-Palmitin-2,3-dilinolein 6.66%1,3-Diolein-2-linolein 16.77% 1-Palmitin-2-linolein-3-olein 12.89%1,3-Dipalmitin-2-linolein 2.88% Triolein 18.30% 1-Palmitin-2,3-diolein10.18%

Process:

Glue formulation: Gelatin, purified water, glycerin and chlorhexidineacetate were weighed at a weight ratio of 1:1.0:0.5:0.008. Glycerin,purified water and chlorhexidine acetate were sequentially added into aglue melting tank and heated to 85. Then gelatin was added andconstantly stirred under vacuum until the gelatin was completelydissolved. The glue was filtered and stored at 56 for use.

Drug liquid formulation: Formulated amount of Coix seed oil and Tween 80were added into an ingredient tank and stirred constantly untilthoroughly mixed.

Capsule pressing: Proper pellet dies were chosen according to thecapsule size. Capsules were pressed under a tempreture of 30 and arelative humidity of less than 35%, then shaped and dried. Afterexcluding capsules of abnormal size, normal capsules were washed with95% medicinal ethanol and dried continuously till the moisture contentwas less than 12%. Unqualified capsules were removed by visualinspection, and the final products were printed and packaged.

Example 27 Preparation of Coix Seed Oil Injection of the Invention

Formulation:

Coix seed oil 100 g Soybean lecithin for injection 10 g Glycerin forinjection 15 g Water for injection adds to 1000 mL

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.42% 1-linolein-3-olein 1.25% 1,2-diolein 0.25%1-olein-2-linolein 0.81% 1,2-dilinolein 0.35% Trilinolein 5.13%1-Olein-2,3-dilinolein 14.09% 1-Palmitin-2,3-dilinolein 5.74%1,3-Diolein-2-linolein 15.01% 1-Palmitin-2-linolein-3-olein 10.95%1,3-Dipalmitin-2-linolein 2.88% Triolein 20.75% 1-Palmitin-2,3-diolein8.69%

Process:

To a formulated amount of soybean lecithin for injection was added anappropriate amount of water for injection. The mixture was dispersedwith a high shear dispersing emulsifier into a dispersion without bulksor granules. Formulated amount of glycerin for injection was added. Thenwater for injection was added to a specified amount, and the mixture wasstirred to give a water phase.

A formulated amount of Coix seed oil was weighed. The weighed oil andthe water phase prepared above were heated separately to 60, then mixedand emulsified in a high pressure homogenizer, in which the low pressurewas 7 MPa and the high pressure was 26 MPa. The homogenization wasrepeated for 5 times until the amount of particles below 2 μm was noless than 95% and particles above 5 μm were undetectable. If necessary,NaOH or HCl was used to adjust the pH to 6.8.

The resulting homogeneous emulsion was filtered by nitrogen pressurethrough a microporous filter of 3 μm or less, then filled undernitrogen, and finally sterilized and cooled to afford the injection.

Example 28 Preparation of Coix Seed Oil Injection of the Invention

Formulation:

Coix seed oil 300 g Soybean lecithin acceptable for injection 40 gGlycerin acceptable for injection 50 g Water for injection adds to 1000mL

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.46% 1-linolein-3-olein 1.20% 1,2-diolein 0.28%1-olein-2-linolein 0.90% 1,2-dilinolein 0.38% Trilinolein 5.71%1-Olein-2,3-dilinolein 15.11% 1-Palmitin-2,3-dilinolein 6.02%1,3-Diolein-2-linolein 15.45% 1-Palmitin-2-linolein-3-olein 14.20%1,3-Dipalmitin-2-linolein 3.20% Triolein 17.14% 1-Palmitin-2,3-diolein9.22%

Process:

To a formulated amount of soybean lecithin acceptable for injection wasadded an appropriate amount of water for injection. The mixture wasdispersed with a high shear dispersing emulsifier into a dispersionwithout bulks or granules. Formulated amount of glycerin acceptable forinjection was added. Then water for injection is added to a specifiedamount, and the mixture was stirred to give a water phase.

A formulated amount of Coix seed oil was weighed. The weighed oil andthe water phase prepared above were heated separately to 70, then mixedand emulsified in a high pressure homogenizer, in which the low pressurewas 11 MPa and the high pressure was 48 MPa. The homogenization wasrepeated for 6 times until the amount of particles below 2 μm was noless than 95% and particles above 5 μm were undetectable. If necessary,NaOH or HCl was used to adjust the pH to 7.5.

The resulting homogeneous emulsion was filtered by nitrogen pressurethrough a microporous filter of 3 μm or less, then filled undernitrogen, and finally sterilized and cooled to afford the injection.

Example 29 Preparation of Coix Seed Oil Injection of the Invention

Formulation:

Coix seed oil 200 g Soybean lecithin for injection 25 g Glycerinacceptable for injection 30 g Water for injection adds to 1000 mL

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.50% 1-linolein-3-olein 1.31% 1,2-diolein 0.30%1-olein-2-linolein 0.95% 1,2-dilinolein 0.41% Trilinolein 6.18%1-Olein-2,3-dilinolein 16.03% 1-Palmitin-2,3-dilinolein 6.51%1,3-Diolein-2-linolein 16.30% 1-Palmitin-2-linolein-3-olein 12.83%1,3-Dipalmitin-2-linolein 2.81% Triolein 18.10% 1-Palmitin-2,3-diolein9.95%

Process:

To a formulated amount of soybean lecithin for injection was added anappropriate amount of water for injection. The mixture was dispersedwith a high shear dispersing emulsifier into a dispersion without bulksor granules. Formulated amount of glycerin acceptable for injection wasadded. Then water for injection is added to a specified amount, and themixture was stirred to give a water phase.

A formulated amount of Coix seed oil was weighed. The weighed oil andthe water phase prepared above were heated separately to 65, then mixedand emulsified in a high pressure homogenizer, in which the low pressurewas 8 MPa and the high pressure was 40 MPa. The homogenization wasrepeated for 4 times until the amount of particles below 2 μm was noless than 95% and particles above 5 μm were undetectable. If necessary,NaOH or HCl was used to adjust the pH to 6.5.

The resulting homogeneous emulsion was filtered by nitrogen pressurethrough a microporous filter of 3 μm or less, then filled undernitrogen, and finally sterilized and cooled to afford the injection.

Example 30 Preparation of Coix Seed Oil Capsule of the Invention

Formulation:

Coix seed oil 200 g Vitamine E 0.20 g to give 1000 capsules

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.52% 1-linolein-3-olein 1.40% 1,2-diolein 0.32%1-olein-2-linolein 1.01% 1,2-dilinolein 0.43% Trilinolein 6.51%1-Olein-2,3-dilinolein 17.26% 1-Palmitin-2,3-dilinolein 6.84%1,3-Diolein-2-linolein 17.65% 1-Palmitin-2-linolein-3-olein 13.56%1,3-Dipalmitin-2-linolein 3.07% Triolein 19.33% 1-Palmitin-2,3-diolein10.80%

Process:

Glue formulation: Gelatin, purified water, glycerin and 10% ethylparabensolution were weighed at a weight ratio of 1:1.2:0.8:0.01. Glycerin,purified water and 10% ethylparaben solution were sequentially addedinto a glue melting tank and heated to 70. Then gelatin was added andconstantly stirred under vacuum until the gelatin was completelydissolved. The glue was filtered and stored at 59 for use.

Drug liquid formulation: Formulated amount of Coix seed oil and VitamineE were added into an ingredient tank and stirred constantly untilthoroughly mixed.

Capsule pressing: Proper pellet dies were chosen according to thecapsule size. Capsules were pressed under a tempreture of 16 and arelative humidity of less than 35%, then shaped and dried. Afterexcluding capsules of abnormal size, normal capsules were washed with95% medicinal ethanol and dried continuously till the moisture contentwas less than 12%. Unqualified capsules were removed by visualinspection, and the final products were printed and packaged.

Example 31 Preparation of Coix Seed Oil Capsule of the Invention

Formulation

Coix seed oil 800 g Tween 80 0.60 g to give 1000 capsules

wherein, the Coix seed oil contains following ingredients

1,3-diolein 0.56% 1-linolein-3-olein 1.11% 1,2-diolein 0.34%1-olein-2-linolein 0.91% 1,2-dilinolein 0.46% Trilinolein 6.71%1-Olein-2,3-dilinolein 18.01% 1-Palmitin-2,3-dilinolein 7.25%1,3-Diolein-2-linolein 18.50% 1-Palmitin-2-linolein-3-olein 11.90%1,3-Dipalmitin-2-linolein 2.63% Triolein 19.91% 1-Palmitin-2,3-diolein11.21%

Process:

Glue formulation: Gelatin, purified water, glycerin and benzoic acidwere weighed at a weight ratio of 1:1.2:0.8:0.01. Glycerin, purifiedwater and benzoic acid were sequentially added into a glue melting tankand heated to 90. Then gelatin was added and constantly stirred undervacuum until the gelatin was completely dissolved. The glue was filteredand stored at 60 for use.

Drug liquid formulation: Formulated amount of Coix seed oil and Tween 80were added into an ingredient tank and stirred constantly untilthoroughly mixed.

Capsule pressing: Proper pellet dies were chosen according to thecapsule size. Capsules were pressed under a tempreture of 26 and arelative humidity of less than 35%, then shaped and dried. Afterexcluding capsules of abnormal size, normal capsules were washed with95% medicinal ethanol and dried continuously till the moisture contentwas less than 12%. Unqualified capsules were removed by visualinspection, and the final products were printed and packaged.

Example 32 Preparation of Coix Seed Oil Capsule of the Invention

Formulation:

Coix seed oil 500 g Vitamine E 0.40 g to give 1000 capsules

wherein, the Coix seed oil contains following ingredients:

1,3-diolein 0.57% 1-linolein-3-olein 1.21% 1,2-diolein 0.34%1-olein-2-linolein 0.86% 1,2-dilinolein 0.46% Trilinolein 6.85%1-Olein-2,3-dilinolein 18.24% 1-Palmitin-2,3-dilinolein 7.25%1,3-Diolein-2-linolein 18.61% 1-Palmitin-2-linolein-3-olein 12.03%1,3-Dipalmitin-2-linolein 3.01% Triolein 18.60% 1-Palmitin-2,3-diolein11.21%

Process:

Glue formulation: Gelatin, purified water, glycerin and potassiumsorbate were weighed at a weight ratio of 1:0.9:0.6:0.005. Glycerin,purified water and potassium sorbate were sequentially added into a gluemelting tank and heated to 80. Then gelatin was added and constantlystirred under vacuum until the gelatin was completely dissolved. Theglue was filtered and stored at 62 for use.

Drug liquid formulation: Formulated amount of Coix seed oil and VitamineE were added into an ingredient tank and stirred constantly untilthoroughly mixed.

Capsule pressing: Proper pellet dies were chosen according to thecapsule size. Capsules were pressed under a tempreture of 20 and arelative humidity of less than 35%, then shaped and dried. Afterexcluding capsules of abnormal size, normal capsules were washed with95% medicinal ethanol and dried continuously till the moisture contentwas less than 12%. Unqualified capsules were removed by visualinspection, and the final products were printed and packaged.

1. A Coix seed oil, comprising 5 diglyceride and 8 triglycerideingredients in the following mass percentages: 1,3-diolein 0.40-0.58%,1-linolein-3-olein 0.91-1.31%, 1,2-diolein 0.24-0.35%,1-olein-2-linolein 0.66-0.95%, 1,2-dilinolein 0.33-0.47%, trilinolein4.87-6.99%, 1-olein-2,3-dilinolein 13.00-18.69%,1-palmitin-2,3-dilinolein 5.25-7.54%, 1,3-diolein-2-linolein13.23-19.02%, 1-palmitin-2-linolein-3-olein 10.26-14.75%,1,3-dipalmitin-2-linolein 2.28-3.28%, triolein 14.44-20.76%, and1-palmitin-2,3-diolein 8.06-11.58%.
 2. The Coix seed oil of claim 1,wherein said 5 diglyceride and 8 triglyceride ingredients are in thefollowing mass percentages: 1,3-diolein 0.45-0.55%, 1-linolein-3-olein1.03-1.25%, 1,2-diolein 0.27-0.33%, 1-olein-2-linolein 0.75-0.91%,1,2-dilinolein 0.37-0.45%, trilinolein 5.47-6.69%,1-olein-2,3-dilinolein 14.63-17.88%, 1-palmitin-2,3-dilinolein5.90-7.21%, 1,3-diolein-2-linolein 14.88-18.19%,1-palmitin-2-linolein-3-olein 11.55-14.11%, 1,3-dipalmitin-2-linolein2.57-3.14%, triolein 16.25-19.86% and 1-palmitin-2,3-diolein9.07-11.08%.
 3. The Coix seed oil of claim 1, wherein said 5 diglycerideand 8 triglyceride ingredients are in the following mass percentages:1,3-diolein 0.49-0.51%, 1-linolein-3-olein 1.12-1.16%, 1,2-diolein0.29-0.31%, 1-olein-2-linolein 0.81-0.85%, 1,2-dilinolein 0.40-0.42%,trilinolein 5.96-6.20%, 1-olein-2,3-dilinolein 15.93-16.58%,1-palmitin-2,3-dilinolein 6.43-6.69%, 1,3-diolein-2-linolein16.20-16.87%, 1-palmitin-2-linolein-3-olein 12.57-13.09%,1,3-dipalmitin-2-linolein 2.79-2.91%, triolein 17.69-18.42% and1-palmitin-2,3-diolein 9.87-10.27%.
 4. The Coix seed oil of claim 1,having the following physicochemical constants based on the fatty oils:specific gravity at 20° C. 0.916-0.920, refractive index at 20° C.1.471-1.474, acid value<0.2, iodine value 100-106, saponification value186-195.
 5. The Coix seed oil of claim 1, which is prepared by arefining process of crude Coix seed oil extracted by supercriticalcarbon dioxide extraction.
 6. The Coix seed oil of claim 5, wherein:said process of the supercritical carbon dioxide extraction comprisessteps of: crushing Coix seeds into 10 mesh-80 mesh powder and extractingsame using a supercritical CO₂ extraction system in which Coix seedpowder is put in 600 L×2 extractors; the CO₂ preheater, extractor andseparation column are heated by jacketed circulating hot water to makethe extraction temperature and separation temperature reach 33-45° C.and 30-45° C., respectively; the outlet temperatures of separator I andseparator II are kept at 20-50° C. and 15-35° C., respectively; theliquid CO₂ is pressed at a flow rate of 1-3 t/h into the CO₂ preheatervia a high pressure pump, turning into a fluid in supercritical state;in the extractor, an oil is extracted into the CO₂ fluid at a pressureof 19-23 Mpa; then the CO₂ fluid with this oil enters the separationcolumn in which the pressure is controlled to 7-10 Mpa to separate thisoil; the CO₂ gas out from the separation column enters sequentially intoseparator I and separator II in which the pressure is sustained at 5-7Mpa and 4-6 Mpa, respectively; impurities such as water separatedtherefrom are discarded; the CO₂ gas returns to liquid CO₂ for reusethrough a condenser; and a continuous extraction for 2-3 h affords thecrude Coix seed oil; and said refining process comprises steps of:adding petroleum ether (bp. 60° C.-90° C.) into the Coix seed oilobtained by the supercritical CO₂ extraction in an amount of 60% of theoil weight; adding 2% NaOH aqueous solution in an amount ranging from36% to 56% of the oil weight according to the acid value; after stirringthe mixture for 10 min and standing for 18-24 h, removing the lowerniger layer; washing the upper layer with purified water and lettingstand for 18-24 h; after the removal of the lower waste water, washingthe upper layer again; after another standing for 40-50 h, removing thelower waste water; and demulsifying the upper layer with acetone in anamount of 70%-90% of the oil weight; after standing for 2-4 h, removingthe lower waste acetone and adding 3 to 8% of activated neutral aluminaby weight of crude oil in the upper oil layer; stirring the mixture for30 min, then filtering off the precipitation; heating the filtrate andadding 2% to 6% of activated kaolin by weight of crude oil; stirring themixture for 30 min at 40-50° C. and then filtering off theprecipitation; concentrating the filtrate under a reduced pressure torecover the solvent, then washing again with purified water; afterstanding for 1-2 h, removing the lower waste water; heating the upperoil layer and vacuum drying it in nitrogen atmosphere; then adding8%-12% activated neutral alumina, stirring the mixture and allowing tostand at a cold place; after filtration, concentrating the filtrated oilvia heating under vacuum in a nitrogen atmosphere and sterilizing thefiltrated oil via dry heat sterilization under vacuum at 160-170° C. for1-2 h; after cooling, filtering the oil through a 0.2 μm microporousmembrane; then split charging the obtained Coix seed oil in 500 mL glassinfusion bottles, nitrogenizing and sealing the bottles.
 7. The Coixseed oil of claim 6, wherein said refining process comprises steps of:adding petroleum ether (bp. 60° C.-90° C.) into the Coix seed oilobtained by the supercritical CO₂ extraction in an amount of 60% of theoil weight; adding 2% NaOH aqueous solution in an amount ranging from36% to 56% of the oil weight according to the acid value; after stirringthe mixture for 10 min and standing for 20 h, removing the lower nigerlayer; washing the upper layer with purified water and letting stand for22 h; after the removal of the lower waste water, washing the upperlayer again; after standing for another 46 h, removing the lower wastewater; demulsifying the upper layer with acetone in an amount of 70%-90%by weight of the crude oil and standing for 3 h; removing the lowerwaste acetone and adding 5% of activated neutral alumina by weight ofcrude oil in the upper oil layer; stirring the mixture for 30 min, thenfiltering off the precipitation; heating the filtrate, and adding 4% ofactivated kaolin by weight of crude oil; stirring the mixture for 30 minat 40-50° C., and then filtering off the precipitation; concentratingthe filtrate under a reduced pressure to recover the solvent, thenwashing again with purified water; after standing for 1 h, removing thelower waste water; heating the upper oil and vacuum drying it undernitrogen atmosphere; then adding 8%-12% activated neutral alumina,stirring the mixture and allowing to stand at a cold place; afterfiltration, concentrating the filtrated oil via heating under vacuum ina nitrogen atmosphere and sterilizing the concentrated oil via dry heatsterilization under vacuum at 160-170° C. for 2 h; after cooling,filtering the oil through a 0.2 μm microporous membrane; then splitcharging the obtained Coix seed oil in 500 mL glass infusion bottles,nitrogenizing and sealing the bottles.
 8. A pharmaceutical preparation,comprising a therapeutically effective amount of the Coix seed oil ofclaim 1 and one or more pharmaceutically acceptable carriers, whereinsaid pharmaceutically acceptable carriers are selected frompharmaceutical conventional dilutions, excipients, fillers, emulsifiers,binders, lubricants, absorption accelerators, surfactants,disintegrants, lubricants and antioxidants, if necessary, flavoringagents, sweeteners, preservatives and/or coloring agents.
 9. Thepharmaceutical preparation of claim 8, wherein said pharmaceuticallyacceptable carriers are selected from one or more in the groupconsisting of: mannitol, sorbitol, sodium metabisulfite, sodiumbisulfite, sodium thiosulfate, cysteine hydrochloride, thioglycolicacid, methionine, soybean lecithin, vitamin C, vitamin E, EDTA disodium,EDTA calcium sodium, a monovalent alkali metal carbonate, acetate,phosphate or its aqueous solution, hydrochloric acid, acetic acid,sulfuric acid, phosphoric acid, amino acids, sodium chloride, potassiumchloride, sodium lactate, ethylparaben solution, benzoic acid, potassiumsorbate, chlorhexidine acetate, xylitol, maltose, glucose, fructose,dextran, glycine, starch, sucrose, lactose, mannitol, and silicicderivatives, cellulose and its derivatives, alginates, gelatin,polyvinyl pyrrolidone, glycerin, Tween 80, agar-agar, calcium carbonate,calcium bicarbonate, surfactant, polyethylene glycol, cyclodextrin,β-cyclodextrin, phospholipid material, kaolin, talc, and calciumstearate or magnesium stearate.
 10. The pharmaceutical preparation ofclaim 8, wherein said pharmaceutical preparation is an oral solidpreparation, an oral liquid preparation, or an injection.
 11. Thepharmaceutical preparation of claim 10, wherein: said oral solidpreparation is selected from any one of capsules, tablets, drippingpills, granules, and concentrated pills; said oral liquid preparation isselected from any one of aqueous or oily suspensions, solutions,emulsions, syrups or elixirs, and a dry product that can bereconstructed by water or other suitable carrier before use; and saidinjection is selected from any one of nano suspensions, liposomes,emulsions, lyophilized powder for injection and aqueous injection. 12.The pharmaceutical preparation of claim 10, wherein said injectioncomprises the following components: Coix seed oil 50-350 g Soybeanlecithin for Injection or 10-40 g soybean lecithin acceptable forinjection Glycerin for Injection or 15-50 g glycerin acceptable forinjection Water for injection adds to 1000 mL


13. The pharmaceutical preparation of claim 12, which is prepared by amethod comprising steps of: adding appropriate amount of water forinjection to a formulated amount of soybean lecithin for injection orsoybean lecithin acceptable for injection; dispersing the mixture with ahigh shear dispersing emulsifier to give a dispersion without bulks orgranules; adding a formulated amount of glycerin for injection orglycerin acceptable for injection; then adding water for injection to aspecified amount, and stirring the mixture to give a water phase;weighing a formulated amount of Coix seed oil; heating the weighed oiland the water phase separately to 60-70° C., then mixing them andemulsifying the mixture in a high pressure homogenizer, in which the lowpressure is 5-12 MPa and the high pressure is 25-50 MPa; repeating thecycle of homogenization for 3-6 times until the amount of particlesbelow 2 μm is no less than 95% and particles above 5 μm areundetectable; if necessary, using NaOH or HCl to adjust the pH to 4.8 to8.5, preferably 6.8 to 7.0, most preferably 6.8; and filtering theresulting homogeneous emulsion by nitrogen pressure through amicroporous filter of 3 μm or less; filling the emulsion, nitrogenizing,sterilizing and cooling to afford the injection.
 14. The pharmaceuticalpreparation of claim 10, wherein said capsule comprises the followingcomponents: Coix seed oil 200-800 g Antioxidant(s) and/or emulsifier(s)0.20-0.60 g to give 1000 capsules


15. The pharmaceutical preparation of claim 14, which is prepared by amethod comprising steps of: preparing glue solution: weighing gelatin,purified water, glycerin and a preservative at a weight ratio of1:0.6-1.2:0.3-0.8:0.0001-0.01; adding glycerin, purified water andpreservative sequentially into a glue melting tank; heating to 70°C.-90° C.; then adding gelatin and constantly stirring the mixture undervacuum until the gelatin is completely dissolved; filtering the gluesolution and storing the filtered glue solution at 56-62° C. for use;preparing drug liquid: adding formulated amount of Coix seed oil,antioxidant(s) and/or emulsifier(s) into an dosing tank, and stirringthe mixture constantly until being homogeneously mixed; and pressingcapsules: choosing proper pellet dies according to the capsule size;pressing capsules in a tempreture of 15-30° C. and a relative humidityof less than 35%; drying the pressed and shaped capsules; after removingcapsules of abnormal size, washing the normal capsules with 95%medicinal ethanol, and drying them continuously till the moisturecontent is less than 12%; visually inspecting and removing unqualifiedcapsules; finally printing and packaging to afford the pharmaceuticalpreparation.
 16. The pharmaceutical preparation of claim 15, wherein:said preservative is selected from any one of 10% ethylparaben solution,benzoic acid, potassium sorbate and chlorhexidine acetate; saidantioxidant is vitamin E; and said emulsifier is Tween
 80. 17. A methodof the treatment of a tumor or an inflammation in a mammal (includinghuman), comprising administering to the mammal (including human) in needa therapeutically effective amount of the Coix seed oil or thepharmaceutical preparation.
 18. The method of claim 17, wherein saidCoix seed oil or said pharmaceutical preparation is administered incombination with a chemotherapeutic drug selected from one or more ofplatinums, alkylating agents, difluoro nucleosides, antibiotics,cytotoxics and/or hormons.
 19. The method of claim 17, wherein said Coixseed oil or said pharmaceutical preparation is administered incombination with a chemotherapeutic drug selected from one or more ofcisplatin, carboplatin, cyclophosphamide, gemcitabine hydrochloride,mitoxantrone, mitomycin, leuprorelin acetate, docetaxel and/orpaclitaxel.
 20. The method of claim 17, wherein said tumor is selectedfrom a group consisting of lung cancer, liver cancer, pancreatic cancer,prostate cancer, ovarian cancer, breast cancer, sarcomatoid carcinomaand cancer sarcoma, in early, middle or late stage; and saidinflammation is prostatitis or prostatic hyperplasia.